Biomarkers of early stage osteoarthritis, rheumatoid arthritis and musculoskeletal health

2.50
Hdl Handle:
http://hdl.handle.net/11287/593908
Title:
Biomarkers of early stage osteoarthritis, rheumatoid arthritis and musculoskeletal health
Authors:
Ahmed, U.; Anwar, A.; Savage, R. S.; Costa, M. L.; Mackay, N.; Filer, A.; Raza, K.; Watts, R. A.; Winyard, Paul G.; Tarr, J.; Haigh, Richard C.; Thornalley, P. J.; Rabbani, N.
Abstract:
There is currently no biochemical test for detection of early-stage osteoarthritis (eOA). Tests for early-stage rheumatoid arthritis (eRA) such as rheumatoid factor (RF) and anti-cyclic citrullinated peptide (CCP) antibodies require refinement to improve clinical utility. We developed robust mass spectrometric methods to quantify citrullinated protein (CP) and free hydroxyproline in body fluids. We detected CP in the plasma of healthy subjects and surprisingly found that CP was increased in both patients with eOA and eRA whereas anti-CCP antibodies were predominantly present in eRA. A 4-class diagnostic algorithm combining plasma/serum CP, anti-CCP antibody and hydroxyproline applied to a cohort gave specific and sensitive detection and discrimination of eOA, eRA, other non-RA inflammatory joint diseases and good skeletal health. This provides a first-in-class plasma/serum-based biochemical assay for diagnosis and type discrimination of early-stage arthritis to facilitate improved treatment and patient outcomes, exploiting citrullinated protein and related differential autoimmunity.
Citation:
Sci Rep. 2015 Mar 19;5:9259.
Publisher:
Nature
Journal:
Scientific reports
Issue Date:
19-Mar-2015
URI:
http://hdl.handle.net/11287/593908
DOI:
10.1038/srep09259
PubMed ID:
25788417
Additional Links:
http://dx.doi.org/10.1038/srep09259
Note:
This article is available via Open Access. Please click on the 'Additional Link' above to access the full-text.
Type:
Journal Article
Language:
eng
ISSN:
2045-2322
Appears in Collections:
2015 RD&E publications; Honorary contracts publications

Full metadata record

DC FieldValue Language
dc.contributor.authorAhmed, U.en
dc.contributor.authorAnwar, A.en
dc.contributor.authorSavage, R. S.en
dc.contributor.authorCosta, M. L.en
dc.contributor.authorMackay, N.en
dc.contributor.authorFiler, A.en
dc.contributor.authorRaza, K.en
dc.contributor.authorWatts, R. A.en
dc.contributor.authorWinyard, Paul G.en
dc.contributor.authorTarr, J.en
dc.contributor.authorHaigh, Richard C.en
dc.contributor.authorThornalley, P. J.en
dc.contributor.authorRabbani, N.en
dc.date.accessioned2016-01-19T12:37:14Zen
dc.date.available2016-01-19T12:37:14Zen
dc.date.issued2015-03-19en
dc.identifier.citationSci Rep. 2015 Mar 19;5:9259.en
dc.identifier.issn2045-2322en
dc.identifier.pmid25788417en
dc.identifier.doi10.1038/srep09259en
dc.identifier.urihttp://hdl.handle.net/11287/593908en
dc.description.abstractThere is currently no biochemical test for detection of early-stage osteoarthritis (eOA). Tests for early-stage rheumatoid arthritis (eRA) such as rheumatoid factor (RF) and anti-cyclic citrullinated peptide (CCP) antibodies require refinement to improve clinical utility. We developed robust mass spectrometric methods to quantify citrullinated protein (CP) and free hydroxyproline in body fluids. We detected CP in the plasma of healthy subjects and surprisingly found that CP was increased in both patients with eOA and eRA whereas anti-CCP antibodies were predominantly present in eRA. A 4-class diagnostic algorithm combining plasma/serum CP, anti-CCP antibody and hydroxyproline applied to a cohort gave specific and sensitive detection and discrimination of eOA, eRA, other non-RA inflammatory joint diseases and good skeletal health. This provides a first-in-class plasma/serum-based biochemical assay for diagnosis and type discrimination of early-stage arthritis to facilitate improved treatment and patient outcomes, exploiting citrullinated protein and related differential autoimmunity.en
dc.language.isoengen
dc.publisherNatureen
dc.relation.urlhttp://dx.doi.org/10.1038/srep09259en
dc.titleBiomarkers of early stage osteoarthritis, rheumatoid arthritis and musculoskeletal healthen
dc.typeJournal Articleen
dc.identifier.journalScientific reportsen
dc.description.noteThis article is available via Open Access. Please click on the 'Additional Link' above to access the full-text.en
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