Age-associated changes in DNA methylation across multiple tissues in an inbred mouse model.

2.50
Hdl Handle:
http://hdl.handle.net/11287/596645
Title:
Age-associated changes in DNA methylation across multiple tissues in an inbred mouse model.
Authors:
Spiers, H; Hannon, Eilis; Wells, S; Williams, B; Fernandes, C; Mill, Jonathan
Abstract:
Epigenetic disruption has been implicated in many diseases of aging, and age-associated DNA methylation changes at specific genomic loci in humans are strongly correlated with chronological age. The aim of this study was to explore the specificity of selected age-associated differentially methylated positions (aDMPs) identified in human epidemiological studies by quantifying DNA methylation across multiple tissues in homologous regions of the murine genome. We selected four high-confidence aDMPs (located in the vicinity of the ELOVL2, GLRA1, MYOD1 and PDE4C genes) and quantified DNA methylation across these regions in four tissues (blood, lung, cerebellum and hippocampus) from male and female C57BL/6J mice, ranging in age from fetal (embryonic day 17) to 630 days. We observed tissue-specific age-associated changes in DNA methylation that was directionally consistent with those observed in humans. These findings lend further support to the notion that changes in DNA methylation are associated with chronological age and suggest that these processes are often conserved across tissues and between mammalian species. Our data highlight the relevance of utilizing model systems, in which environmental and genetic influences can be carefully controlled, for the further study of these phenomena.
Citation:
Age-associated changes in DNA methylation across multiple tissues in an inbred mouse model. 2016: Mech. Ageing Dev.
Publisher:
Elsevier
Journal:
Mechanisms of ageing and development
Issue Date:
6-Feb-2016
URI:
http://hdl.handle.net/11287/596645
DOI:
10.1016/j.mad.2016.02.001
PubMed ID:
26861500
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0047-6374(16)30006-9
Note:
The article is freely available via Open Access. Click on the 'Additional Link' above to access the full-text from the publisher's site.
Type:
Comment
Language:
en
ISSN:
1872-6216
Appears in Collections:
Honorary contracts publications; 2016 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorSpiers, Hen
dc.contributor.authorHannon, Eilisen
dc.contributor.authorWells, Sen
dc.contributor.authorWilliams, Ben
dc.contributor.authorFernandes, Cen
dc.contributor.authorMill, Jonathanen
dc.date.accessioned2016-02-18T15:24:14Zen
dc.date.available2016-02-18T15:24:14Zen
dc.date.issued2016-02-06en
dc.identifier.citationAge-associated changes in DNA methylation across multiple tissues in an inbred mouse model. 2016: Mech. Ageing Dev.en
dc.identifier.issn1872-6216en
dc.identifier.pmid26861500en
dc.identifier.doi10.1016/j.mad.2016.02.001en
dc.identifier.urihttp://hdl.handle.net/11287/596645en
dc.description.abstractEpigenetic disruption has been implicated in many diseases of aging, and age-associated DNA methylation changes at specific genomic loci in humans are strongly correlated with chronological age. The aim of this study was to explore the specificity of selected age-associated differentially methylated positions (aDMPs) identified in human epidemiological studies by quantifying DNA methylation across multiple tissues in homologous regions of the murine genome. We selected four high-confidence aDMPs (located in the vicinity of the ELOVL2, GLRA1, MYOD1 and PDE4C genes) and quantified DNA methylation across these regions in four tissues (blood, lung, cerebellum and hippocampus) from male and female C57BL/6J mice, ranging in age from fetal (embryonic day 17) to 630 days. We observed tissue-specific age-associated changes in DNA methylation that was directionally consistent with those observed in humans. These findings lend further support to the notion that changes in DNA methylation are associated with chronological age and suggest that these processes are often conserved across tissues and between mammalian species. Our data highlight the relevance of utilizing model systems, in which environmental and genetic influences can be carefully controlled, for the further study of these phenomena.en
dc.languageENGen
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0047-6374(16)30006-9en
dc.rightsArchived with thanks to Mechanisms of ageing and developmenten
dc.subjectWessex Classification Subject Headings::Oncology. Pathology.::Geneticsen
dc.titleAge-associated changes in DNA methylation across multiple tissues in an inbred mouse model.en
dc.typeCommenten
dc.identifier.journalMechanisms of ageing and developmenten
dc.description.noteThe article is freely available via Open Access. Click on the 'Additional Link' above to access the full-text from the publisher's site.en
dc.type.versionIn press (epub ahead of print)en

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