Red Blood Cell Susceptibility to Pneumolysin: Correlation with Membrane Biochemical and Physical Properties.

2.50
Hdl Handle:
http://hdl.handle.net/11287/603580
Title:
Red Blood Cell Susceptibility to Pneumolysin: Correlation with Membrane Biochemical and Physical Properties.
Authors:
Bokori-Brown, M.; Petrov, P. G.; Khafaji, M. A.; Mughal, M. K.; Naylor, C. E.; Shore, Angela ( 0000-0003-3039-308x ) ; Gooding, Kim M.; Casanova, Francesco; Mitchell, T. J.; Titball, R. W.; Winlove, C. P.
Abstract:
This study investigated the effect of the biochemical and biophysical properties of the plasma membrane as well as membrane morphology on the susceptibility of human red blood cells to the cholesterol-dependent cytolysin pneumolysin, a key virulence factor of Streptococcus pneumoniae, using single cell studies. We show a correlation between the physical properties of the membrane (bending rigidity, surface and dipole electrostatic potentials) and the susceptibility of red blood cells to pneumolysin-induced haemolysis. We demonstrate that biochemical modifications of the membrane induced by oxidative stress, lipid scrambling and artificial cell aging modulate the cell response to the toxin. We provide evidence that the diversity of response to pneumolysin in diabetic red blood cells correlates with levels of glycated haemoglobin (Hba1c) and that the mechanical properties of the red blood cell plasma membrane are altered in diabetes. Finally, we show that diabetic red blood cells are more resistant to pneumolysin and the related toxin perfringolysin O relative to healthy red blood cells. Taken together, these studies indicate that the diversity of cell response to pneumolysin within a population of human red blood cells is influenced by the biophysical and biochemical status of the plasma membrane and the chemical and/or oxidative stress prehistory of the cell.
Citation:
Red Blood Cell Susceptibility to Pneumolysin: Correlation with Membrane Biochemical and Physical Properties. 2016 May 6;291(19):10210-27
Publisher:
Journal of Biological Chemistry
Journal:
The Journal of biological chemistry
Issue Date:
16-Mar-2016
URI:
http://hdl.handle.net/11287/603580
DOI:
10.1074/jbc.M115.691899
PubMed ID:
26984406
Additional Links:
http://www.jbc.org/cgi/pmidlookup?view=long&pmid=26984406
Note:
This article is freely available from the publisher's website. Click on the 'Additional Link' above to access the publisher's site.
Type:
Journal Article
Language:
en
ISSN:
1083-351X
Appears in Collections:
Cardiology; Honorary contracts publications; 2016 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorBokori-Brown, M.en
dc.contributor.authorPetrov, P. G.en
dc.contributor.authorKhafaji, M. A.en
dc.contributor.authorMughal, M. K.en
dc.contributor.authorNaylor, C. E.en
dc.contributor.authorShore, Angelaen
dc.contributor.authorGooding, Kim M.en
dc.contributor.authorCasanova, Francescoen
dc.contributor.authorMitchell, T. J.en
dc.contributor.authorTitball, R. W.en
dc.contributor.authorWinlove, C. P.en
dc.date.accessioned2016-03-23T16:52:43Zen
dc.date.available2016-03-23T16:52:43Zen
dc.date.issued2016-03-16en
dc.identifier.citationRed Blood Cell Susceptibility to Pneumolysin: Correlation with Membrane Biochemical and Physical Properties. 2016 May 6;291(19):10210-27en
dc.identifier.issn1083-351Xen
dc.identifier.pmid26984406en
dc.identifier.doi10.1074/jbc.M115.691899en
dc.identifier.urihttp://hdl.handle.net/11287/603580en
dc.description.abstractThis study investigated the effect of the biochemical and biophysical properties of the plasma membrane as well as membrane morphology on the susceptibility of human red blood cells to the cholesterol-dependent cytolysin pneumolysin, a key virulence factor of Streptococcus pneumoniae, using single cell studies. We show a correlation between the physical properties of the membrane (bending rigidity, surface and dipole electrostatic potentials) and the susceptibility of red blood cells to pneumolysin-induced haemolysis. We demonstrate that biochemical modifications of the membrane induced by oxidative stress, lipid scrambling and artificial cell aging modulate the cell response to the toxin. We provide evidence that the diversity of response to pneumolysin in diabetic red blood cells correlates with levels of glycated haemoglobin (Hba1c) and that the mechanical properties of the red blood cell plasma membrane are altered in diabetes. Finally, we show that diabetic red blood cells are more resistant to pneumolysin and the related toxin perfringolysin O relative to healthy red blood cells. Taken together, these studies indicate that the diversity of cell response to pneumolysin within a population of human red blood cells is influenced by the biophysical and biochemical status of the plasma membrane and the chemical and/or oxidative stress prehistory of the cell.en
dc.languageENGen
dc.language.isoenen
dc.publisherJournal of Biological Chemistryen
dc.relation.urlhttp://www.jbc.org/cgi/pmidlookup?view=long&pmid=26984406en
dc.rightsArchived with thanks to The Journal of biological chemistryen
dc.subjectWessex Classification Subject Headings::Endocrinology::Diabetesen
dc.titleRed Blood Cell Susceptibility to Pneumolysin: Correlation with Membrane Biochemical and Physical Properties.en
dc.typeJournal Articleen
dc.identifier.journalThe Journal of biological chemistryen
dc.description.noteThis article is freely available from the publisher's website. Click on the 'Additional Link' above to access the publisher's site.en
dc.type.versionIn press (epub ahead of print)en

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