Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function.

2.50
Hdl Handle:
http://hdl.handle.net/11287/606044
Title:
Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function.
Authors:
McDonald, Timothy J. ( 0000-0003-3559-6660 ) ; Perry, Mandy ( 0000-0003-4303-2718 )
Abstract:
C-peptide is a protein secreted by the pancreatic beta cells in equimolar quantities with insulin, following the cleavage of proinsulin into insulin. Measurement of C-peptide is used as a surrogate marker of endogenous insulin secretory capacity. Assessing C-peptide levels can be useful in classifying the subtype of diabetes as well as assessing potential treatment choices in the management of diabetes.Standard measures of C-peptide involve blood samples collected either fasted or, most often, after a fixed stimulus (such as oral glucose, mixed meal, or IV glucagon). Despite the established clinical utility of blood C-peptide measurement, its widespread use is limited. In many instances this is due to perceived practical restrictions associated with sample collection.Urine C-peptide measurement is an attractive noninvasive alternative to blood measures of beta-cell function. Urine C-peptide creatinine ratio measured in a single post stimulated sample has been shown to be a robust, reproducible measure of endogenous C-peptide which is stable for three days at room temperature when collected in boric acid. Modern high sensitivity immunoassay technologies have facilitated measurement of C-peptide down to single picomolar concentrations.
Citation:
Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function. 2016;1433:93-102: Methods Mol. Biol.
Publisher:
Springer
Journal:
Methods in molecular biology
Issue Date:
16-Apr-2016
URI:
http://hdl.handle.net/11287/606044
DOI:
10.1007/7651_2016_330
PubMed ID:
27083170
Additional Links:
http://dx.doi.org/10.1007/7651_2016_330
Type:
Journal Article
Language:
en
ISSN:
1940-6029
Appears in Collections:
Exeter Clinical Laboratory International (Blood Sciences, Genetics, Cellular Pathology & Microbiology); 2016 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorMcDonald, Timothy J.en
dc.contributor.authorPerry, Mandyen
dc.date.accessioned2016-04-20T10:12:46Zen
dc.date.available2016-04-20T10:12:46Zen
dc.date.issued2016-04-16en
dc.identifier.citationDetection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function. 2016;1433:93-102: Methods Mol. Biol.en
dc.identifier.issn1940-6029en
dc.identifier.pmid27083170en
dc.identifier.doi10.1007/7651_2016_330en
dc.identifier.urihttp://hdl.handle.net/11287/606044en
dc.description.abstractC-peptide is a protein secreted by the pancreatic beta cells in equimolar quantities with insulin, following the cleavage of proinsulin into insulin. Measurement of C-peptide is used as a surrogate marker of endogenous insulin secretory capacity. Assessing C-peptide levels can be useful in classifying the subtype of diabetes as well as assessing potential treatment choices in the management of diabetes.Standard measures of C-peptide involve blood samples collected either fasted or, most often, after a fixed stimulus (such as oral glucose, mixed meal, or IV glucagon). Despite the established clinical utility of blood C-peptide measurement, its widespread use is limited. In many instances this is due to perceived practical restrictions associated with sample collection.Urine C-peptide measurement is an attractive noninvasive alternative to blood measures of beta-cell function. Urine C-peptide creatinine ratio measured in a single post stimulated sample has been shown to be a robust, reproducible measure of endogenous C-peptide which is stable for three days at room temperature when collected in boric acid. Modern high sensitivity immunoassay technologies have facilitated measurement of C-peptide down to single picomolar concentrations.en
dc.languageENGen
dc.language.isoenen
dc.publisherSpringeren
dc.relation.urlhttp://dx.doi.org/10.1007/7651_2016_330en
dc.rightsArchived with thanks to Methods in molecular biology. This is the accepted version of the manuscript, the final publication is available at: http://dx.doi.org/10.1007/7651_2016_330en
dc.subjectWessex Classification Subject Headings::Clinical pathologyen
dc.titleDetection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function.en
dc.typeJournal Articleen
dc.identifier.journalMethods in molecular biologyen
dc.type.versionIn press (epub ahead of print)en

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