Exendin-4 protected against critical limb ischemia in obese mice.

2.50
Hdl Handle:
http://hdl.handle.net/11287/610877
Title:
Exendin-4 protected against critical limb ischemia in obese mice.
Authors:
Sheu, J-J; Chang, M-W; Wallace, Christopher G ( 0000-0003-1897-9520 ) ; Chiang, H-J; Sung, P-H; Tsai, T-H; Chung, S-Y; Chen, Y-L; Chua, S; Chang, H-W; Sun, C-K; Lee, F-Y; Yip, H-K
Abstract:
This study tested the hypothesis that exendin-4 protects against critical limb ischemia (CLI) in obese mice undergoing hypoxic stress (H). B6 mice were categorized into aged-matched control (C)-H (group 1-A), obesity (induced by high-fat diet) (O)-H (group 1-B), C-H-CLI (group 2-A), O-H-CLI (group 2-B), C-H-CLI-exendin-4 (group 3-A) and O-H-CLI-exendin-4 (group 3-B). Animals were sacrificed by day 14 after CLI procedure. By day 14, laser Doppler results showed that blood flow in CLI area was higher in group 3-A than group 2-A, higher in group 3-B than group 2-B, highest in groups 1-A and 1-B, higher in group 2-A than in group 2-B, and higher in group 3-A than in group 3-B (all p<0.001), but not significantly different between groups 1-A and 1-B. Furthermore, circulating numbers of endothelial progenitor cells (EPCs) (c-kit/CD31+, Sca-1/KDR+) showed an identical pattern of blood flow in CLI area among groups 2-A, 2-B, 3-A and 3-B, except that these biomarkers were lowest in groups 1-A and 1-B (all p<0.001). Protein and cellular levels of angiogenesis factors (VEGF, CXCR4, SDF-1α) exhibited an identical pattern of circulating EPC numbers among all groups (all p<0.001). Protein levels of apoptotic (cytosolic cytochrome-C, mitochondrial Bax, cleaved caspase 3 and PARP) and fibrotic (Samd 3, TGF-β) biomarkers showed an opposite pattern of blood flow in CLI area among groups 2-A, 2-B, 3-A and 3-B, but were lowest in groups 1-A and 1-B (all p<0.001). This finding suggests exendin-4 protected against CLI in obese mice undergoing hypoxic stress mainly through enhancing angiogenesis and inhibiting apoptosis.
Citation:
Exendin-4 protected against critical limb ischemia in obese mice. 2015, 7 (3):445-59 Am J Transl Res
Publisher:
e-Century Publishing
Journal:
American journal of translational research
Issue Date:
15-Mar-2015
URI:
http://hdl.handle.net/11287/610877
PubMed ID:
26045886
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26045886/
Note:
The full-text of this article is free via PubMed Central. Click on the 'Additional Link' above to access the full-text.
Type:
Journal Article
Language:
en
ISSN:
1943-8141
Appears in Collections:
2015 RD&E publications; Plastic & Reconstructive Surgery

Full metadata record

DC FieldValue Language
dc.contributor.authorSheu, J-Jen
dc.contributor.authorChang, M-Wen
dc.contributor.authorWallace, Christopher Gen
dc.contributor.authorChiang, H-Jen
dc.contributor.authorSung, P-Hen
dc.contributor.authorTsai, T-Hen
dc.contributor.authorChung, S-Yen
dc.contributor.authorChen, Y-Len
dc.contributor.authorChua, Sen
dc.contributor.authorChang, H-Wen
dc.contributor.authorSun, C-Ken
dc.contributor.authorLee, F-Yen
dc.contributor.authorYip, H-Ken
dc.date.accessioned2016-05-27T10:44:30Z-
dc.date.available2016-05-27T10:44:30Z-
dc.date.issued2015-03-15-
dc.identifier.citationExendin-4 protected against critical limb ischemia in obese mice. 2015, 7 (3):445-59 Am J Transl Resen
dc.identifier.issn1943-8141-
dc.identifier.pmid26045886-
dc.identifier.urihttp://hdl.handle.net/11287/610877-
dc.description.abstractThis study tested the hypothesis that exendin-4 protects against critical limb ischemia (CLI) in obese mice undergoing hypoxic stress (H). B6 mice were categorized into aged-matched control (C)-H (group 1-A), obesity (induced by high-fat diet) (O)-H (group 1-B), C-H-CLI (group 2-A), O-H-CLI (group 2-B), C-H-CLI-exendin-4 (group 3-A) and O-H-CLI-exendin-4 (group 3-B). Animals were sacrificed by day 14 after CLI procedure. By day 14, laser Doppler results showed that blood flow in CLI area was higher in group 3-A than group 2-A, higher in group 3-B than group 2-B, highest in groups 1-A and 1-B, higher in group 2-A than in group 2-B, and higher in group 3-A than in group 3-B (all p<0.001), but not significantly different between groups 1-A and 1-B. Furthermore, circulating numbers of endothelial progenitor cells (EPCs) (c-kit/CD31+, Sca-1/KDR+) showed an identical pattern of blood flow in CLI area among groups 2-A, 2-B, 3-A and 3-B, except that these biomarkers were lowest in groups 1-A and 1-B (all p<0.001). Protein and cellular levels of angiogenesis factors (VEGF, CXCR4, SDF-1α) exhibited an identical pattern of circulating EPC numbers among all groups (all p<0.001). Protein levels of apoptotic (cytosolic cytochrome-C, mitochondrial Bax, cleaved caspase 3 and PARP) and fibrotic (Samd 3, TGF-β) biomarkers showed an opposite pattern of blood flow in CLI area among groups 2-A, 2-B, 3-A and 3-B, but were lowest in groups 1-A and 1-B (all p<0.001). This finding suggests exendin-4 protected against CLI in obese mice undergoing hypoxic stress mainly through enhancing angiogenesis and inhibiting apoptosis.en
dc.language.isoenen
dc.publishere-Century Publishingen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26045886/en
dc.rightsArchived with thanks to American journal of translational researchen
dc.subjectWessex Classification Subject Headings::Neurology::Strokeen
dc.titleExendin-4 protected against critical limb ischemia in obese mice.en
dc.typeJournal Articleen
dc.identifier.journalAmerican journal of translational researchen
dc.description.noteThe full-text of this article is free via PubMed Central. Click on the 'Additional Link' above to access the full-text.en
dc.type.versionPublisheden

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