The relationship between cleavage, DNA replication, and gene expression in the mouse 2-cell embryo.

2.50
Hdl Handle:
http://hdl.handle.net/11287/615965
Title:
The relationship between cleavage, DNA replication, and gene expression in the mouse 2-cell embryo.
Authors:
Bolton, V.N.; Oades, Patrick ( 0000-0001-5265-6923 ) ; Johnson, M.H.
Abstract:
The 2-cell stage of mouse embryogenesis is characterized by two phases of alpha-amanitin-sensitive polypeptide synthetic activity, which appear to mark the first major expression of the embryonic genome, as assessed by examination of in vitro translates of mRNA. Using populations of embryos synchronized to the first cleavage division, we have established that DNA replication takes place over the period 1 to 5.5 h after the first cleavage division; the two bursts of putative transcription take place before and immediately after DNA replication, and the translation products are detectable in each case within 3-4 h. In addition, we have shown that suppression of cytokinesis and the second round of DNA replication does not affect synthesis of the alpha-amanitin-sensitive polypeptides, and that neither DNA replication nor the loss of maternal mRNA that take place during the 2-cell stage are dependent upon synthesis of the alpha-amanitin-sensitive polypeptides.
Citation:
The relationship between cleavage, DNA replication, and gene expression in the mouse 2-cell embryo. 1984, 79:139-63 J Embryol Exp Morphol
Publisher:
Development
Journal:
Journal of embryology and experimental morphology
Issue Date:
Feb-1984
URI:
http://hdl.handle.net/11287/615965
PubMed ID:
6716041
Additional Links:
http://dev.biologists.org/content/79/1/139.long
Note:
This article is freely available via Open Access. Click on the ‘Additional Link’ above to access the full-text from the publisher’s site.
Type:
Research Support, Non-U.S. Gov't
Language:
en
ISSN:
0022-0752
Appears in Collections:
pre-2014 RD&E publications; Paediatrics

Full metadata record

DC FieldValue Language
dc.contributor.authorBolton, V.N.en
dc.contributor.authorOades, Patricken
dc.contributor.authorJohnson, M.H.en
dc.date.accessioned2016-07-12T13:45:06Z-
dc.date.available2016-07-12T13:45:06Z-
dc.date.issued1984-02-
dc.identifier.citationThe relationship between cleavage, DNA replication, and gene expression in the mouse 2-cell embryo. 1984, 79:139-63 J Embryol Exp Morpholen
dc.identifier.issn0022-0752-
dc.identifier.pmid6716041-
dc.identifier.urihttp://hdl.handle.net/11287/615965-
dc.description.abstractThe 2-cell stage of mouse embryogenesis is characterized by two phases of alpha-amanitin-sensitive polypeptide synthetic activity, which appear to mark the first major expression of the embryonic genome, as assessed by examination of in vitro translates of mRNA. Using populations of embryos synchronized to the first cleavage division, we have established that DNA replication takes place over the period 1 to 5.5 h after the first cleavage division; the two bursts of putative transcription take place before and immediately after DNA replication, and the translation products are detectable in each case within 3-4 h. In addition, we have shown that suppression of cytokinesis and the second round of DNA replication does not affect synthesis of the alpha-amanitin-sensitive polypeptides, and that neither DNA replication nor the loss of maternal mRNA that take place during the 2-cell stage are dependent upon synthesis of the alpha-amanitin-sensitive polypeptides.en
dc.language.isoenen
dc.publisherDevelopmenten
dc.relation.urlhttp://dev.biologists.org/content/79/1/139.longen
dc.rightsArchived with thanks to Journal of embryology and experimental morphologyen
dc.subjectWessex Classification Subject Headings::Oncology. Pathology.::Geneticsen
dc.titleThe relationship between cleavage, DNA replication, and gene expression in the mouse 2-cell embryo.en
dc.typeResearch Support, Non-U.S. Gov'ten
dc.identifier.journalJournal of embryology and experimental morphologyen
dc.description.noteThis article is freely available via Open Access. Click on the ‘Additional Link’ above to access the full-text from the publisher’s site.en
dc.type.versionPublisheden
All Items in RD&E Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.