Additional benefit of combined therapy with melatonin and apoptotic adipose-derived mesenchymal stem cell against sepsis-induced kidney injury.

2.50
Hdl Handle:
http://hdl.handle.net/11287/618070
Title:
Additional benefit of combined therapy with melatonin and apoptotic adipose-derived mesenchymal stem cell against sepsis-induced kidney injury.
Authors:
Chen, H-H; Lin, K-C; Wallace, Christopher G ( 0000-0003-1897-9520 ) ; Chen, Y-T; Yang, C-C; Leu, S; Chen, Y-C; Sun, C-K; Tsai, T-H; Chen, Y-L; Chung, S-Y; Chang, C-L; Yip, H-K
Abstract:
This study tested whether combined therapy with melatonin and apoptotic adipose-derived mesenchymal stem cells (A-ADMSCs) offered additional benefit in ameliorating sepsis-induced acute kidney injury. Adult male Sprague-Dawley rats (n = 65) were randomized equally into five groups: Sham controls (SC), sepsis induced by cecal-ligation and puncture (CLP), CLP-melatonin, CLP-A-ADMSC, and CLP-melatonin-A-ADMSC. Circulating TNF-α level at post-CLP 6 hr was highest in CLP and lowest in SC groups, higher in CLP-melatonin than in CLP-A-ADMSC and CLP-melatonin-A-ADMSC groups (all P < 0.001). Immune reactivity as reflected in the number of splenic helper-, cytoxic-, and regulatory-T cells at post-CLP 72 hr exhibited the same pattern as that of circulating TNF-α among all groups (P < 0.001). The histological scoring of kidney injury and the number of F4/80+ and CD14+ cells in kidney were highest in CLP and lowest in SC groups, higher in CLP-melatonin than in CLP-A-ADMSC and CLP-melatonin-A-ADMSC groups, and higher in CLP-A-ADMSC than in CLP-melatonin-A-ADMSC groups (all P < 0.001). Changes in protein expressions of inflammatory (RANTES, TNF-1α, NF-κB, MMP-9, MIP-1, IL-1β), apoptotic (cleaved caspase 3 and PARP, mitochondrial Bax), fibrotic (Smad3, TGF-β) markers, reactive-oxygen-species (NOX-1, NOX-2), and oxidative stress displayed a pattern identical to that of kidney injury score among the five groups (all P < 0.001). Expressions of antioxidants (GR+, GPx+, HO-1, NQO-1+) were lowest in SC group and highest in CLP-melatonin-A-ADMSC group, lower in CLP than in CLP-melatonin and CLP-A-ADMSC groups, and lower in CLP-melatonin- than in CLP-A-ADMSC-tretaed animals (all P < 0.001). In conclusion, combined treatment with melatonin and A-ADMSC was superior to A-ADMSC alone in protecting the kidneys from sepsis-induced injury.
Citation:
Additional benefit of combined therapy with melatonin and apoptotic adipose-derived mesenchymal stem cell against sepsis-induced kidney injury. 2014, 57 (1):16-32 J. Pineal Res.
Publisher:
Wiley
Journal:
Journal of pineal research
Issue Date:
Aug-2014
URI:
http://hdl.handle.net/11287/618070
DOI:
10.1111/jpi.12140
PubMed ID:
24761983
Additional Links:
http://dx.doi.org/10.1111/jpi.12140
Type:
Journal Article; Research Support, Non-U.S. Gov't
Language:
en
ISSN:
1600-079X
Appears in Collections:
Plastic & Reconstructive Surgery; 2014 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, H-Hen
dc.contributor.authorLin, K-Cen
dc.contributor.authorWallace, Christopher Gen
dc.contributor.authorChen, Y-Ten
dc.contributor.authorYang, C-Cen
dc.contributor.authorLeu, Sen
dc.contributor.authorChen, Y-Cen
dc.contributor.authorSun, C-Ken
dc.contributor.authorTsai, T-Hen
dc.contributor.authorChen, Y-Len
dc.contributor.authorChung, S-Yen
dc.contributor.authorChang, C-Len
dc.contributor.authorYip, H-Ken
dc.date.accessioned2016-08-09T10:28:40Z-
dc.date.available2016-08-09T10:28:40Z-
dc.date.issued2014-08-
dc.identifier.citationAdditional benefit of combined therapy with melatonin and apoptotic adipose-derived mesenchymal stem cell against sepsis-induced kidney injury. 2014, 57 (1):16-32 J. Pineal Res.en
dc.identifier.issn1600-079X-
dc.identifier.pmid24761983-
dc.identifier.doi10.1111/jpi.12140-
dc.identifier.urihttp://hdl.handle.net/11287/618070-
dc.description.abstractThis study tested whether combined therapy with melatonin and apoptotic adipose-derived mesenchymal stem cells (A-ADMSCs) offered additional benefit in ameliorating sepsis-induced acute kidney injury. Adult male Sprague-Dawley rats (n = 65) were randomized equally into five groups: Sham controls (SC), sepsis induced by cecal-ligation and puncture (CLP), CLP-melatonin, CLP-A-ADMSC, and CLP-melatonin-A-ADMSC. Circulating TNF-α level at post-CLP 6 hr was highest in CLP and lowest in SC groups, higher in CLP-melatonin than in CLP-A-ADMSC and CLP-melatonin-A-ADMSC groups (all P < 0.001). Immune reactivity as reflected in the number of splenic helper-, cytoxic-, and regulatory-T cells at post-CLP 72 hr exhibited the same pattern as that of circulating TNF-α among all groups (P < 0.001). The histological scoring of kidney injury and the number of F4/80+ and CD14+ cells in kidney were highest in CLP and lowest in SC groups, higher in CLP-melatonin than in CLP-A-ADMSC and CLP-melatonin-A-ADMSC groups, and higher in CLP-A-ADMSC than in CLP-melatonin-A-ADMSC groups (all P < 0.001). Changes in protein expressions of inflammatory (RANTES, TNF-1α, NF-κB, MMP-9, MIP-1, IL-1β), apoptotic (cleaved caspase 3 and PARP, mitochondrial Bax), fibrotic (Smad3, TGF-β) markers, reactive-oxygen-species (NOX-1, NOX-2), and oxidative stress displayed a pattern identical to that of kidney injury score among the five groups (all P < 0.001). Expressions of antioxidants (GR+, GPx+, HO-1, NQO-1+) were lowest in SC group and highest in CLP-melatonin-A-ADMSC group, lower in CLP than in CLP-melatonin and CLP-A-ADMSC groups, and lower in CLP-melatonin- than in CLP-A-ADMSC-tretaed animals (all P < 0.001). In conclusion, combined treatment with melatonin and A-ADMSC was superior to A-ADMSC alone in protecting the kidneys from sepsis-induced injury.en
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttp://dx.doi.org/10.1111/jpi.12140en
dc.rightsArchived with thanks to Journal of pineal researchen
dc.subjectWessex Classification Subject Headings::Urology::Nephrology/Renal medicineen
dc.subjectWessex Classification Subject Headings::Surgery::Plastic surgeryen
dc.titleAdditional benefit of combined therapy with melatonin and apoptotic adipose-derived mesenchymal stem cell against sepsis-induced kidney injury.en
dc.typeJournal Articleen
dc.typeResearch Support, Non-U.S. Gov'ten
dc.identifier.journalJournal of pineal researchen
dc.type.versionPublisheden

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