A novel rat full-thickness hemi-abdominal wall/hindlimb osteomyocutaneous combined flap: influence of allograft mass and vascularized bone marrow content on vascularized composite allograft survival.

2.50
Hdl Handle:
http://hdl.handle.net/11287/618097
Title:
A novel rat full-thickness hemi-abdominal wall/hindlimb osteomyocutaneous combined flap: influence of allograft mass and vascularized bone marrow content on vascularized composite allograft survival.
Authors:
Ramirez, A. E.; Cheng, H-Y; Lao, W. W.; Wang, Y-L; Wen, C-J; Wallace, Christopher G ( 0000-0003-1897-9520 ) ; Lin, C-F; Shih, L-Y; Chuang, S-H; Wei, F-C
Abstract:
Vascularized bone marrow transplantation (VBMT) appears to promote tolerance for vascularized composite allotransplantation (VCA). However, it is unclear whether VBMT is critical for tolerance induction and, if so, whether there is a finite amount of VCA that VBMT can support. We investigated this with a novel VCA combined flap model incorporating full-thickness hemiabdominal wall and hindlimb osteomyocutaneous (HAW/HLOMC) flaps. Effects of allograft mass (AM) and VBMT on VCA outcome were studied by comparing HAW/HLOMC VCAs with fully MHC-mismatched BN donors and Lewis recipients. Control groups did not receive treatments following transplantation. Treatment groups received a short course of cyclosporine A (CsA), antilymphocyte serum, and three doses of adipocyte-derived stem cells (POD 1, 8, and 15). The results showed that all flaps in control allogeneic groups rejected soon after VCAs. Treatment significantly prolonged allograft survival. Three of eight recipients in HLOMC treatment group had allografts survive long-term and developed donor-specific tolerance. Significantly higher peripheral chimerism was observed in HLOMC than other groups. It is concluded that the relative amount of AM to VBMT is a critical factor influencing long-term allograft survival. Accordingly, VBMT content compared with VCA mass may be an important consideration for VCA in humans.
Citation:
A novel rat full-thickness hemi-abdominal wall/hindlimb osteomyocutaneous combined flap: influence of allograft mass and vascularized bone marrow content on vascularized composite allograft survival. 2014, 27 (9):977-86 Transpl. Int.
Publisher:
Wiley
Journal:
Transplant international : official journal of the European Society for Organ Transplantation
Issue Date:
Sep-2014
URI:
http://hdl.handle.net/11287/618097
DOI:
10.1111/tri.12364
PubMed ID:
24861714
Additional Links:
http://dx.doi.org/10.1111/tri.12364
Note:
This article is freely available from the publisher's site. Click on the 'Additional Link' above to access the full-text.
Type:
Journal Article; Research Support, Non-U.S. Gov't
Language:
en
ISSN:
1432-2277
Appears in Collections:
Plastic & Reconstructive Surgery; 2014 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorRamirez, A. E.en
dc.contributor.authorCheng, H-Yen
dc.contributor.authorLao, W. W.en
dc.contributor.authorWang, Y-Len
dc.contributor.authorWen, C-Jen
dc.contributor.authorWallace, Christopher Gen
dc.contributor.authorLin, C-Fen
dc.contributor.authorShih, L-Yen
dc.contributor.authorChuang, S-Hen
dc.contributor.authorWei, F-Cen
dc.date.accessioned2016-08-09T10:14:51Z-
dc.date.available2016-08-09T10:14:51Z-
dc.date.issued2014-09-
dc.identifier.citationA novel rat full-thickness hemi-abdominal wall/hindlimb osteomyocutaneous combined flap: influence of allograft mass and vascularized bone marrow content on vascularized composite allograft survival. 2014, 27 (9):977-86 Transpl. Int.en
dc.identifier.issn1432-2277-
dc.identifier.pmid24861714-
dc.identifier.doi10.1111/tri.12364-
dc.identifier.urihttp://hdl.handle.net/11287/618097-
dc.description.abstractVascularized bone marrow transplantation (VBMT) appears to promote tolerance for vascularized composite allotransplantation (VCA). However, it is unclear whether VBMT is critical for tolerance induction and, if so, whether there is a finite amount of VCA that VBMT can support. We investigated this with a novel VCA combined flap model incorporating full-thickness hemiabdominal wall and hindlimb osteomyocutaneous (HAW/HLOMC) flaps. Effects of allograft mass (AM) and VBMT on VCA outcome were studied by comparing HAW/HLOMC VCAs with fully MHC-mismatched BN donors and Lewis recipients. Control groups did not receive treatments following transplantation. Treatment groups received a short course of cyclosporine A (CsA), antilymphocyte serum, and three doses of adipocyte-derived stem cells (POD 1, 8, and 15). The results showed that all flaps in control allogeneic groups rejected soon after VCAs. Treatment significantly prolonged allograft survival. Three of eight recipients in HLOMC treatment group had allografts survive long-term and developed donor-specific tolerance. Significantly higher peripheral chimerism was observed in HLOMC than other groups. It is concluded that the relative amount of AM to VBMT is a critical factor influencing long-term allograft survival. Accordingly, VBMT content compared with VCA mass may be an important consideration for VCA in humans.en
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttp://dx.doi.org/10.1111/tri.12364en
dc.rightsArchived with thanks to Transplant international : official journal of the European Society for Organ Transplantationen
dc.subjectWessex Classification Subject Headings::Surgery::Plastic surgeryen
dc.titleA novel rat full-thickness hemi-abdominal wall/hindlimb osteomyocutaneous combined flap: influence of allograft mass and vascularized bone marrow content on vascularized composite allograft survival.en
dc.typeJournal Articleen
dc.typeResearch Support, Non-U.S. Gov'ten
dc.identifier.journalTransplant international : official journal of the European Society for Organ Transplantationen
dc.description.noteThis article is freely available from the publisher's site. Click on the 'Additional Link' above to access the full-text.en
dc.type.versionPublisheden

Related articles on PubMed

All Items in RD&E Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.