Analysis of transcription factors key for mouse pancreatic development establishes NKX2-2 and MNX1 mutations as causes of neonatal diabetes in man.

2.50
Hdl Handle:
http://hdl.handle.net/11287/618265
Title:
Analysis of transcription factors key for mouse pancreatic development establishes NKX2-2 and MNX1 mutations as causes of neonatal diabetes in man.
Authors:
Flanagan, Sarah; De Franco, Elisa; Lango Allen, H.; Zerah, M.; Abdul-Rasoul, M. M.; Edge, J.A.; Stewart, H.; Alamiri, E.; Hussain, K.; Wallis, Sa.; de Vries, L.; Rubio-Cabezas, O.; Houghton, J. A. L.; Edghill, E. L.; Patch, A-M; Ellard, Sian ( 0000-0002-7620-5526 ) ; Hattersley, Andrew T.
Abstract:
Understanding transcriptional regulation of pancreatic development is required to advance current efforts in developing beta cell replacement therapies for patients with diabetes. Current knowledge of key transcriptional regulators has predominantly come from mouse studies, with rare, naturally occurring mutations establishing their relevance in man. This study used a combination of homozygosity analysis and Sanger sequencing in 37 consanguineous patients with permanent neonatal diabetes to search for homozygous mutations in 29 transcription factor genes important for murine pancreatic development. We identified homozygous mutations in 7 different genes in 11 unrelated patients and show that NKX2-2 and MNX1 are etiological genes for neonatal diabetes, thus confirming their key role in development of the human pancreas. The similar phenotype of the patients with recessive mutations and mice with inactivation of a transcription factor gene support there being common steps critical for pancreatic development and validate the use of rodent models for beta cell development.
Citation:
Analysis of transcription factors key for mouse pancreatic development establishes NKX2-2 and MNX1 mutations as causes of neonatal diabetes in man. 2014, 19 (1):146-54 Cell Metab.
Publisher:
Cell Press
Journal:
Cell Metabolism
Issue Date:
7-Jan-2014
URI:
http://hdl.handle.net/11287/618265
DOI:
10.1016/j.cmet.2013.11.021
PubMed ID:
24411943
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S1550-4131(13)00492-0
Note:
This article is freely available via Open Access. Click on the 'Additional Link' above to access the full text.
Type:
Journal Article; Research Support, Non-U.S. Gov't
Language:
en
ISSN:
1932-7420
Appears in Collections:
2014 RD&E publications; Diabetes/Endocrine Services; Molecular Genetics; Honorary contracts publications

Full metadata record

DC FieldValue Language
dc.contributor.authorFlanagan, Sarahen
dc.contributor.authorDe Franco, Elisaen
dc.contributor.authorLango Allen, H.en
dc.contributor.authorZerah, M.en
dc.contributor.authorAbdul-Rasoul, M. M.en
dc.contributor.authorEdge, J.A.en
dc.contributor.authorStewart, H.en
dc.contributor.authorAlamiri, E.en
dc.contributor.authorHussain, K.en
dc.contributor.authorWallis, Sa.en
dc.contributor.authorde Vries, L.en
dc.contributor.authorRubio-Cabezas, O.en
dc.contributor.authorHoughton, J. A. L.en
dc.contributor.authorEdghill, E. L.en
dc.contributor.authorPatch, A-Men
dc.contributor.authorEllard, Sianen
dc.contributor.authorHattersley, Andrew T.en
dc.date.accessioned2016-08-11T15:25:42Z-
dc.date.available2016-08-11T15:25:42Z-
dc.date.issued2014-01-07-
dc.identifier.citationAnalysis of transcription factors key for mouse pancreatic development establishes NKX2-2 and MNX1 mutations as causes of neonatal diabetes in man. 2014, 19 (1):146-54 Cell Metab.en
dc.identifier.issn1932-7420-
dc.identifier.pmid24411943-
dc.identifier.doi10.1016/j.cmet.2013.11.021-
dc.identifier.urihttp://hdl.handle.net/11287/618265-
dc.description.abstractUnderstanding transcriptional regulation of pancreatic development is required to advance current efforts in developing beta cell replacement therapies for patients with diabetes. Current knowledge of key transcriptional regulators has predominantly come from mouse studies, with rare, naturally occurring mutations establishing their relevance in man. This study used a combination of homozygosity analysis and Sanger sequencing in 37 consanguineous patients with permanent neonatal diabetes to search for homozygous mutations in 29 transcription factor genes important for murine pancreatic development. We identified homozygous mutations in 7 different genes in 11 unrelated patients and show that NKX2-2 and MNX1 are etiological genes for neonatal diabetes, thus confirming their key role in development of the human pancreas. The similar phenotype of the patients with recessive mutations and mice with inactivation of a transcription factor gene support there being common steps critical for pancreatic development and validate the use of rodent models for beta cell development.en
dc.language.isoenen
dc.publisherCell Pressen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S1550-4131(13)00492-0en
dc.rightsArchived with thanks to Cell metabolismen
dc.subjectWessex Classification Subject Headings::Oncology. Pathology.::Geneticsen
dc.subjectWessex Classification Subject Headings::Endocrinology::Diabetesen
dc.titleAnalysis of transcription factors key for mouse pancreatic development establishes NKX2-2 and MNX1 mutations as causes of neonatal diabetes in man.en
dc.typeJournal Articleen
dc.typeResearch Support, Non-U.S. Gov'ten
dc.identifier.journalCell Metabolismen
dc.description.noteThis article is freely available via Open Access. Click on the 'Additional Link' above to access the full text.en
dc.type.versionPublisheden

Related articles on PubMed

All Items in RD&E Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.