Directed evolution of aldolases for exploitation in synthetic organic chemistry.

2.50
Hdl Handle:
http://hdl.handle.net/11287/619038
Title:
Directed evolution of aldolases for exploitation in synthetic organic chemistry.
Authors:
Bolt, Amanda H. ( 0000-0003-4303-2718 ) ; Berry, A.; Nelson, A.
Abstract:
This review focuses on the directed evolution of aldolases with synthetically useful properties. Directed evolution has been used to address a number of limitations associated with the use of wild-type aldolases as catalysts in synthetic organic chemistry. The generation of aldolase enzymes with a modified or expanded substrate repertoire is described. Particular emphasis is placed on the directed evolution of aldolases with modified stereochemical properties: such enzymes can be useful catalysts in the stereoselective synthesis of biologically active small molecules. The review also describes some of the fundamental insights into mechanistic enzymology that directed evolution can provide.
Citation:
Directed evolution of aldolases for exploitation in synthetic organic chemistry. 2008, 474 (2):318-30 Arch. Biochem. Biophys.
Publisher:
Elsevier
Journal:
Archives of biochemistry and biophysics
Issue Date:
15-Jun-2008
URI:
http://hdl.handle.net/11287/619038
DOI:
10.1016/j.abb.2008.01.005
PubMed ID:
18230325
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0003986108000167
Note:
This article is freely available via Open Access. Click on the 'Additional Link' above to access the full-text from the publisher's site.
Type:
Research Support, Non-U.S. Gov't
Language:
en
ISSN:
1096-0384
Appears in Collections:
pre-2014 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorBolt, Amanda H.en
dc.contributor.authorBerry, A.en
dc.contributor.authorNelson, A.en
dc.date.accessioned2016-08-30T10:01:28Z-
dc.date.available2016-08-30T10:01:28Z-
dc.date.issued2008-06-15-
dc.identifier.citationDirected evolution of aldolases for exploitation in synthetic organic chemistry. 2008, 474 (2):318-30 Arch. Biochem. Biophys.en
dc.identifier.issn1096-0384-
dc.identifier.pmid18230325-
dc.identifier.doi10.1016/j.abb.2008.01.005-
dc.identifier.urihttp://hdl.handle.net/11287/619038-
dc.description.abstractThis review focuses on the directed evolution of aldolases with synthetically useful properties. Directed evolution has been used to address a number of limitations associated with the use of wild-type aldolases as catalysts in synthetic organic chemistry. The generation of aldolase enzymes with a modified or expanded substrate repertoire is described. Particular emphasis is placed on the directed evolution of aldolases with modified stereochemical properties: such enzymes can be useful catalysts in the stereoselective synthesis of biologically active small molecules. The review also describes some of the fundamental insights into mechanistic enzymology that directed evolution can provide.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0003986108000167en
dc.rightsArchived with thanks to Archives of biochemistry and biophysicsen
dc.subjectWessex Classification Subject Headings::Biochemistryen
dc.titleDirected evolution of aldolases for exploitation in synthetic organic chemistry.en
dc.typeResearch Support, Non-U.S. Gov'ten
dc.identifier.journalArchives of biochemistry and biophysicsen
dc.description.noteThis article is freely available via Open Access. Click on the 'Additional Link' above to access the full-text from the publisher's site.en
dc.description.fundingBB/E000622/1/Biotechnology and Biological Sciences Research Council/United Kingdom BBS/B/05680/Biotechnology and Biological Sciences Research Council/United Kingdom Biotechnology and Biological Sciences Research Council/United Kingdom Wellcome Trust/United Kingdomen
dc.type.versionPublisheden

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