Quality of placental RNA: Effects of explant size and culture duration.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620046
Title:
Quality of placental RNA: Effects of explant size and culture duration.
Authors:
Brew, O; Nikolopoulou, E; Hughes, A (RD&E); Christian, M; Lee, Y; Oduwole, O; Sullivan, M H F; Woodman, A
Abstract:
We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided.
Citation:
Quality of placental RNA: Effects of explant size and culture duration. 2016, 46:45-48 Placenta
Publisher:
Elsevier
Journal:
Placenta
Issue Date:
Oct-2016
URI:
http://hdl.handle.net/11287/620046
DOI:
10.1016/j.placenta.2016.08.083
PubMed ID:
27697221
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0143-4004(16)30489-1
Type:
Journal Article
Language:
en
ISSN:
1532-3102
Appears in Collections:
Exeter Clinical Laboratory International (Blood Sciences, Genetics, Cellular Pathology & Microbiology); 2016 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorBrew, Oen
dc.contributor.authorNikolopoulou, Een
dc.contributor.authorHughes, A (RD&E)en
dc.contributor.authorChristian, Men
dc.contributor.authorLee, Yen
dc.contributor.authorOduwole, Oen
dc.contributor.authorSullivan, M H Fen
dc.contributor.authorWoodman, Aen
dc.date.accessioned2016-10-19T10:01:15Z-
dc.date.available2016-10-19T10:01:15Z-
dc.date.issued2016-10-
dc.identifier.citationQuality of placental RNA: Effects of explant size and culture duration. 2016, 46:45-48 Placentaen
dc.identifier.issn1532-3102-
dc.identifier.pmid27697221-
dc.identifier.doi10.1016/j.placenta.2016.08.083-
dc.identifier.urihttp://hdl.handle.net/11287/620046-
dc.description.abstractWe evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided.en
dc.languageENG-
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0143-4004(16)30489-1en
dc.rightsArchived with thanks to Placentaen
dc.subjectWessex Classification Subject Headings::Microbiology. Immunologyen
dc.titleQuality of placental RNA: Effects of explant size and culture duration.en
dc.typeJournal Articleen
dc.identifier.journalPlacentaen
dc.type.versionPublisheden

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