Melatonin treatment improves adipose-derived mesenchymal stem cell therapy for acute lung ischemia-reperfusion injury

2.50
Hdl Handle:
http://hdl.handle.net/11287/620125
Title:
Melatonin treatment improves adipose-derived mesenchymal stem cell therapy for acute lung ischemia-reperfusion injury
Authors:
Yip, H-K; Chang, Y-C; Wallace, Christopher G ( 0000-0003-1897-9520 ) ; Chang, L-T; Tsai, T-H; Chen, Y-L; Chang, H-W; Leu, S; Zhen, Y-Y; Tsai, C-Y; Yeh, K-H; Sun, C-K; Yen, C-H
Abstract:
This study investigated whether melatonin-treated adipose-derived mesenchymal stem cells (ADMSC) offered superior protection against acute lung ischemia-reperfusion (IR) injury. Adult male Sprague-Dawley rats (n = 30) were randomized equally into five groups: sham controls, lung IR-saline, lung IR-melatonin, lung IR-melatonin-normal ADMSC, and lung IR-melatonin-apoptotic ADMSC. Arterial oxygen saturation was lowest in lung IR-saline; lower in lung IR-melatonin than sham controls, lung IR-melatonin-normal ADMSC, and lung IR-melatonin-apoptotic ADMSC; lower in lung IR-melatonin-normal ADMSC than sham controls and lung IR-melatonin-apoptotic ADMSC; lower in lung IR-melatonin-apoptotic ADMSC than sham controls (P < 0.0001 in each case). Right ventricular systolic blood pressure (RVSBP) showed a reversed pattern among all groups (all P < 0.0001). Changes in histological scoring of lung parenchymal damage and CD68+ cells showed a similar pattern compared with RVSBP in all groups (all P < 0.001). Changes in inflammatory protein expressions such as VCAM-1, ICAM-1, oxidative stress, TNF-α, NF-κB, PDGF, and angiotensin II receptor, and changes in apoptotic protein expressions of cleaved caspase 3 and PARP, and mitochondrial Bax, displayed identical patterns compared with RVSBP in all groups (all P < 0.001). Numbers of antioxidant (GR+, GPx+, NQO-1+) and endothelial cell biomarkers (CD31+ and vWF+) were lower in sham controls, lung IR-saline, and lung IR-melatonin than lung IR-melatonin-normal ADMSC and lung IR-melatonin-apoptotic ADMSC, and lower in lung IR-melatonin-normal ADMSC than lung IR-melatonin-apoptotic ADMSC (P < 0.001 in each case). In conclusion, when the animals were treated with melatonin, the apoptotic ADMSC were superior to normal ADMSC for protection of lung from acute IR injury.
Citation:
Melatonin treatment improves adipose-derived mesenchymal stem cell therapy for acute lung ischemia-reperfusion injury 2013, 54 (2):207 Journal of Pineal Research
Publisher:
Wiley
Journal:
Journal of Pineal Research
Issue Date:
Mar-2013
URI:
http://hdl.handle.net/11287/620125
DOI:
10.1111/jpi.12020
Additional Links:
http://doi.wiley.com/10.1111/jpi.12020
Type:
Journal Article; Research Support, Non-U.S. Gov't
Language:
en
ISSN:
07423098
Appears in Collections:
Plastic & Reconstructive Surgery; pre-2014 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorYip, H-Ken
dc.contributor.authorChang, Y-Cen
dc.contributor.authorWallace, Christopher Gen
dc.contributor.authorChang, L-Ten
dc.contributor.authorTsai, T-Hen
dc.contributor.authorChen, Y-Len
dc.contributor.authorChang, H-Wen
dc.contributor.authorLeu, Sen
dc.contributor.authorZhen, Y-Yen
dc.contributor.authorTsai, C-Yen
dc.contributor.authorYeh, K-Hen
dc.contributor.authorSun, C-Ken
dc.contributor.authorYen, C-Hen
dc.date.accessioned2017-01-04T15:41:18Z-
dc.date.available2017-01-04T15:41:18Z-
dc.date.issued2013-03-
dc.identifier.citationMelatonin treatment improves adipose-derived mesenchymal stem cell therapy for acute lung ischemia-reperfusion injury 2013, 54 (2):207 Journal of Pineal Researchen
dc.identifier.issn07423098-
dc.identifier.doi10.1111/jpi.12020-
dc.identifier.urihttp://hdl.handle.net/11287/620125-
dc.description.abstractThis study investigated whether melatonin-treated adipose-derived mesenchymal stem cells (ADMSC) offered superior protection against acute lung ischemia-reperfusion (IR) injury. Adult male Sprague-Dawley rats (n = 30) were randomized equally into five groups: sham controls, lung IR-saline, lung IR-melatonin, lung IR-melatonin-normal ADMSC, and lung IR-melatonin-apoptotic ADMSC. Arterial oxygen saturation was lowest in lung IR-saline; lower in lung IR-melatonin than sham controls, lung IR-melatonin-normal ADMSC, and lung IR-melatonin-apoptotic ADMSC; lower in lung IR-melatonin-normal ADMSC than sham controls and lung IR-melatonin-apoptotic ADMSC; lower in lung IR-melatonin-apoptotic ADMSC than sham controls (P < 0.0001 in each case). Right ventricular systolic blood pressure (RVSBP) showed a reversed pattern among all groups (all P < 0.0001). Changes in histological scoring of lung parenchymal damage and CD68+ cells showed a similar pattern compared with RVSBP in all groups (all P < 0.001). Changes in inflammatory protein expressions such as VCAM-1, ICAM-1, oxidative stress, TNF-α, NF-κB, PDGF, and angiotensin II receptor, and changes in apoptotic protein expressions of cleaved caspase 3 and PARP, and mitochondrial Bax, displayed identical patterns compared with RVSBP in all groups (all P < 0.001). Numbers of antioxidant (GR+, GPx+, NQO-1+) and endothelial cell biomarkers (CD31+ and vWF+) were lower in sham controls, lung IR-saline, and lung IR-melatonin than lung IR-melatonin-normal ADMSC and lung IR-melatonin-apoptotic ADMSC, and lower in lung IR-melatonin-normal ADMSC than lung IR-melatonin-apoptotic ADMSC (P < 0.001 in each case). In conclusion, when the animals were treated with melatonin, the apoptotic ADMSC were superior to normal ADMSC for protection of lung from acute IR injury.en
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttp://doi.wiley.com/10.1111/jpi.12020en
dc.rightsArchived with thanks to Journal of Pineal Researchen
dc.subjectWessex Classification Subject Headings::Respiratory medicineen
dc.titleMelatonin treatment improves adipose-derived mesenchymal stem cell therapy for acute lung ischemia-reperfusion injuryen
dc.typeJournal Articleen
dc.typeResearch Support, Non-U.S. Gov'ten
dc.identifier.journalJournal of Pineal Researchen
dc.type.versionPublisheden
dc.contributor.institutionDivision of Cardiology; Department of Internal Medicine; Xiamen Chang Gung Memorial Hospital; Fujian; China-
dc.contributor.institutionDepartment of Plastic Surgery; University Hospital of South Manchester; Manchester; UK-
dc.contributor.institutionNursing Department; Basic Science; Meiho University; Pingtung; Taiwan-
dc.contributor.institutionDivision of Cardiology; Department of Internal Medicine; Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine; Kaohsiung; Taiwan-
dc.contributor.institutionDivision of Cardiology; Department of Internal Medicine; Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine; Kaohsiung; Taiwan-
dc.contributor.institutionDepartment of Biological Sciences; National Sun Yat-Sen University; Kaohsiung; Taiwan-
dc.contributor.institutionCenter for Translational Research in Biomedical Sciences; Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine; Kaohsiung; Taiwan-
dc.contributor.institutionDivision of Cardiology; Department of Internal Medicine; Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine; Kaohsiung; Taiwan-
dc.contributor.institutionInstitute of Molecular Biology; Academia Sinica; Taipei; Taiwan-
dc.contributor.institutionDivision of Cardiology; Department of Internal Medicine; Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine; Kaohsiung; Taiwan-
dc.contributor.institutionDepartment of Emergency Medicine; E-DA Hospital; I-Shou University; Kaohsiung; Taiwan-
dc.contributor.institutionDepartment of Biological Science and Technology; National Pingtung University of Science and Technology; Pingtung; Taiwan-
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