Current laboratory requirements for adrenocorticotropic hormone and renin/aldosterone sample handling are unnecessarily restrictive.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620214
Title:
Current laboratory requirements for adrenocorticotropic hormone and renin/aldosterone sample handling are unnecessarily restrictive.
Authors:
Chakera, A. J.; McDonald, Timothy J. ( 0000-0003-3559-6660 ) ; Knight, B. A.; Vaidya, Bijay; Jones, A.G.
Abstract:
Samples for adrenocorticotropic hormone (ACTH) and aldosterone/renin analysis usually require rapid transport to the receiving laboratory for immediate separation and freezing. In practice, this means assessment is limited to hospital settings and many samples are rejected. We examined whether these requirements are necessary by assessing the stability of ACTH, aldosterone and renin over 48 hours in whole blood collected in serum gel and EDTA plasma from 31 participants. Our results show that ACTH collected into EDTA plasma is stable at room temperature for at least 6 hours, mean change at 6 hours -2.6% (95% CI -9.7 to 4.5). Both aldosterone and renin were stable collected on serum gel at room temperature for at least 6 hours: mean change aldosterone +0.2% (95% CI -3.6 to 4.0), renin -1.9% (95% CI -7.0 to3.2). Therefore, by using appropriate preservatives, ACTH and aldosterone/renin can be measured on samples collected at room temperature and processed within 6 hours. This would facilitate outpatient and emergency room assessment of these analytes.
Citation:
Current laboratory requirements for adrenocorticotropic hormone and renin/aldosterone sample handling are unnecessarily restrictive. 2017, 17 (1):18-21 Clin Med (Lond)
Publisher:
Royal College of Physicians
Journal:
Clinical Medicine (London, England)
Issue Date:
Feb-2017
URI:
http://hdl.handle.net/11287/620214
DOI:
10.7861/clinmedicine.17-1-18
PubMed ID:
28148573
Additional Links:
https://dx.doi.org/10.7861/clinmedicine.17-1-18
Type:
Journal Article
Language:
en
ISSN:
1473-4893
Appears in Collections:
Exeter Clinical Laboratory International (Blood Sciences, Genetics, Cellular Pathology & Microbiology); 2017 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorChakera, A. J.en
dc.contributor.authorMcDonald, Timothy J.en
dc.contributor.authorKnight, B. A.en
dc.contributor.authorVaidya, Bijayen
dc.contributor.authorJones, A.G.en
dc.date.accessioned2017-02-06T16:17:15Z-
dc.date.available2017-02-06T16:17:15Z-
dc.date.issued2017-02-
dc.identifier.citationCurrent laboratory requirements for adrenocorticotropic hormone and renin/aldosterone sample handling are unnecessarily restrictive. 2017, 17 (1):18-21 Clin Med (Lond)en
dc.identifier.issn1473-4893-
dc.identifier.pmid28148573-
dc.identifier.doi10.7861/clinmedicine.17-1-18-
dc.identifier.urihttp://hdl.handle.net/11287/620214-
dc.description.abstractSamples for adrenocorticotropic hormone (ACTH) and aldosterone/renin analysis usually require rapid transport to the receiving laboratory for immediate separation and freezing. In practice, this means assessment is limited to hospital settings and many samples are rejected. We examined whether these requirements are necessary by assessing the stability of ACTH, aldosterone and renin over 48 hours in whole blood collected in serum gel and EDTA plasma from 31 participants. Our results show that ACTH collected into EDTA plasma is stable at room temperature for at least 6 hours, mean change at 6 hours -2.6% (95% CI -9.7 to 4.5). Both aldosterone and renin were stable collected on serum gel at room temperature for at least 6 hours: mean change aldosterone +0.2% (95% CI -3.6 to 4.0), renin -1.9% (95% CI -7.0 to3.2). Therefore, by using appropriate preservatives, ACTH and aldosterone/renin can be measured on samples collected at room temperature and processed within 6 hours. This would facilitate outpatient and emergency room assessment of these analytes.en
dc.language.isoenen
dc.publisherRoyal College of Physiciansen
dc.relation.urlhttps://dx.doi.org/10.7861/clinmedicine.17-1-18en
dc.rightsArchived with thanks to Clinical Medicine (London, England)en
dc.subjectWessex Classification Subject Headings::Microbiology. Immunologyen
dc.titleCurrent laboratory requirements for adrenocorticotropic hormone and renin/aldosterone sample handling are unnecessarily restrictive.en
dc.typeJournal Articleen
dc.identifier.journalClinical Medicine (London, England)en
dc.type.versionPublisheden

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