Intravenous administration of xenogenic adipose-derived mesenchymal stem cells (ADMSC) and ADMSC-derived exosomes markedly reduced brain infarct volume and preserved neurological function in rat after acute ischemic stroke.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620233
Title:
Intravenous administration of xenogenic adipose-derived mesenchymal stem cells (ADMSC) and ADMSC-derived exosomes markedly reduced brain infarct volume and preserved neurological function in rat after acute ischemic stroke.
Authors:
Chen, K-H; Chen, C-H; Wallace, Christopher G ( 0000-0003-1897-9520 ) ; Yuen, C-M; Kao, G-S; Chen, Y-L; Shao, P-L; Chen, Y-L; Chai, H-T; Lin, K-C; Liu, C-F; Chang, H-W; Lee, M. S.; Yip, H-K
Abstract:
We tested the hypothesis that combined xenogenic (from mini-pig) adipose-derived mesenchymal stem cell (ADMSC) and ADMSC-derived exosome therapy could reduce brain-infarct zone (BIZ) and enhance neurological recovery in rat after acute ischemic stroke (AIS) induced by 50-min left middle cerebral artery occlusion. Adult-male Sprague-Dawley rats (n = 60) were divided equally into group 1 (sham-control), group 2 (AIS), group 3 [AIS-ADMSC (1.2×106 cells)], group 4 [AIS-exosome (100μg)], and group 5 (AIS-exosome-ADMSC). All therapies were provided intravenously at 3h after AIS procedure. BIZ determined by histopathology (by day-60) and brain MRI (by day-28) were highest in group 2, lowest in group 1, higher in groups 3 and 4 than in group 5, but they showed no difference between groups 3 and 4 (all p < 0.0001). By day-28, sensorimotor functional results exhibited an opposite pattern to BIZ among the five groups (p < 0.005). Protein expressions of inflammatory (inducible nitric oxide synthase/tumor necrosis factor-α/nuclear factor-κB/interleukin-1β/matrix metalloproteinase-9/plasminogen activator inhibitor-1/RANTES), oxidative-stress (NOX-1/NOX-2/oxidized protein), apoptotic (caspase-3/ Poly-ADP-ribose polymerase), and fibrotic (Smad3/transforming growth factor-β) biomarkers, and cellular expressions of brain-damaged (γ-H2AX+/ XRCC1-CD90+/p53BP1-CD90+), inflammatory (CD11+/CD68+/glial fibrillary acid protein+) and brain-edema (aquaporin-4+) markers showed a similar pattern of BIZ among the groups (all n < 0.0001). In conclusion, xenogenic ADMSC/ADMSC-derived exosome therapy was safe and offered the additional benefit of reducing BIZ and improving neurological function in rat AIS.
Citation:
Intravenous administration of xenogenic adipose-derived mesenchymal stem cells (ADMSC) and ADMSC-derived exosomes markedly reduced brain infarct volume and preserved neurological function in rat after acute ischemic stroke. 2016, 7 (46):74537-74556 Oncotarget
Publisher:
Oncotarget
Journal:
Oncotarget
Issue Date:
15-Nov-2016
URI:
http://hdl.handle.net/11287/620233
DOI:
10.18632/oncotarget.12902
PubMed ID:
27793019
Additional Links:
http://www.impactjournals.com/oncotarget/misc/linkedout.php?pii=12902
Note:
This article is freely available online. Click on the Additional Link above to access the full-text.
Type:
Journal Article
Language:
en
ISSN:
1949-2553
Appears in Collections:
Plastic & Reconstructive Surgery; 2016 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, K-Hen
dc.contributor.authorChen, C-Hen
dc.contributor.authorWallace, Christopher Gen
dc.contributor.authorYuen, C-Men
dc.contributor.authorKao, G-Sen
dc.contributor.authorChen, Y-Len
dc.contributor.authorShao, P-Len
dc.contributor.authorChen, Y-Len
dc.contributor.authorChai, H-Ten
dc.contributor.authorLin, K-Cen
dc.contributor.authorLiu, C-Fen
dc.contributor.authorChang, H-Wen
dc.contributor.authorLee, M. S.en
dc.contributor.authorYip, H-Ken
dc.date.accessioned2017-03-02T11:02:51Z-
dc.date.available2017-03-02T11:02:51Z-
dc.date.issued2016-11-15-
dc.identifier.citationIntravenous administration of xenogenic adipose-derived mesenchymal stem cells (ADMSC) and ADMSC-derived exosomes markedly reduced brain infarct volume and preserved neurological function in rat after acute ischemic stroke. 2016, 7 (46):74537-74556 Oncotargeten
dc.identifier.issn1949-2553-
dc.identifier.pmid27793019-
dc.identifier.doi10.18632/oncotarget.12902-
dc.identifier.urihttp://hdl.handle.net/11287/620233-
dc.description.abstractWe tested the hypothesis that combined xenogenic (from mini-pig) adipose-derived mesenchymal stem cell (ADMSC) and ADMSC-derived exosome therapy could reduce brain-infarct zone (BIZ) and enhance neurological recovery in rat after acute ischemic stroke (AIS) induced by 50-min left middle cerebral artery occlusion. Adult-male Sprague-Dawley rats (n = 60) were divided equally into group 1 (sham-control), group 2 (AIS), group 3 [AIS-ADMSC (1.2×106 cells)], group 4 [AIS-exosome (100μg)], and group 5 (AIS-exosome-ADMSC). All therapies were provided intravenously at 3h after AIS procedure. BIZ determined by histopathology (by day-60) and brain MRI (by day-28) were highest in group 2, lowest in group 1, higher in groups 3 and 4 than in group 5, but they showed no difference between groups 3 and 4 (all p < 0.0001). By day-28, sensorimotor functional results exhibited an opposite pattern to BIZ among the five groups (p < 0.005). Protein expressions of inflammatory (inducible nitric oxide synthase/tumor necrosis factor-α/nuclear factor-κB/interleukin-1β/matrix metalloproteinase-9/plasminogen activator inhibitor-1/RANTES), oxidative-stress (NOX-1/NOX-2/oxidized protein), apoptotic (caspase-3/ Poly-ADP-ribose polymerase), and fibrotic (Smad3/transforming growth factor-β) biomarkers, and cellular expressions of brain-damaged (γ-H2AX+/ XRCC1-CD90+/p53BP1-CD90+), inflammatory (CD11+/CD68+/glial fibrillary acid protein+) and brain-edema (aquaporin-4+) markers showed a similar pattern of BIZ among the groups (all n < 0.0001). In conclusion, xenogenic ADMSC/ADMSC-derived exosome therapy was safe and offered the additional benefit of reducing BIZ and improving neurological function in rat AIS.en
dc.language.isoenen
dc.publisherOncotargeten
dc.relation.urlhttp://www.impactjournals.com/oncotarget/misc/linkedout.php?pii=12902en
dc.rightsArchived with thanks to Oncotargeten
dc.subjectWessex Classification Subject Headings::Surgery::Plastic surgeryen
dc.titleIntravenous administration of xenogenic adipose-derived mesenchymal stem cells (ADMSC) and ADMSC-derived exosomes markedly reduced brain infarct volume and preserved neurological function in rat after acute ischemic stroke.en
dc.typeJournal Articleen
dc.identifier.journalOncotargeten
dc.description.noteThis article is freely available online. Click on the Additional Link above to access the full-text.en
dc.type.versionPublisheden

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