Mapping epigenetic changes to the host cell genome induced by Burkholderia pseudomallei reveals pathogen-specific and pathogen-generic signatures of infection.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620271
Title:
Mapping epigenetic changes to the host cell genome induced by Burkholderia pseudomallei reveals pathogen-specific and pathogen-generic signatures of infection.
Authors:
Cizmeci, D.; Dempster, E. L.; Champion, O. L.; Wagley, S.; Akman, O. E.; Prior, J. L.; Soyer, O. S.; Mill, Jonathan; Titball, R. W.
Abstract:
The potential for epigenetic changes in host cells following microbial infection has been widely suggested, but few examples have been reported. We assessed genome-wide patterns of DNA methylation in human macrophage-like U937 cells following infection with Burkholderia pseudomallei, an intracellular bacterial pathogen and the causative agent of human melioidosis. Our analyses revealed significant changes in host cell DNA methylation, at multiple CpG sites in the host cell genome, following infection. Infection induced differentially methylated probes (iDMPs) showing the greatest changes in DNA methylation were found to be in the vicinity of genes involved in inflammatory responses, intracellular signalling, apoptosis and pathogen-induced signalling. A comparison of our data with reported methylome changes in cells infected with M. tuberculosis revealed commonality of differentially methylated genes, including genes involved in T cell responses (BCL11B, FOXO1, KIF13B, PAWR, SOX4, SYK), actin cytoskeleton organisation (ACTR3, CDC42BPA, DTNBP1, FERMT2, PRKCZ, RAC1), and cytokine production (FOXP1, IRF8, MR1). Overall our findings show that pathogenic-specific and pathogen-common changes in the methylome occur following infection.
Citation:
Mapping epigenetic changes to the host cell genome induced by Burkholderia pseudomallei reveals pathogen-specific and pathogen-generic signatures of infection. 2016, 6:30861 Sci Rep
Publisher:
Nature
Journal:
Scientific reports
Issue Date:
3-Aug-2016
URI:
http://hdl.handle.net/11287/620271
DOI:
10.1038/srep30861
PubMed ID:
27484700
Additional Links:
http://dx.doi.org/10.1038/srep30861
Note:
This article is freely available. Click on the Additional Link above to access the full-text.
Type:
Journal Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
Honorary contracts publications; 2016 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorCizmeci, D.en
dc.contributor.authorDempster, E. L.en
dc.contributor.authorChampion, O. L.en
dc.contributor.authorWagley, S.en
dc.contributor.authorAkman, O. E.en
dc.contributor.authorPrior, J. L.en
dc.contributor.authorSoyer, O. S.en
dc.contributor.authorMill, Jonathanen
dc.contributor.authorTitball, R. W.en
dc.date.accessioned2017-03-13T10:08:58Z-
dc.date.available2017-03-13T10:08:58Z-
dc.date.issued2016-08-03-
dc.identifier.citationMapping epigenetic changes to the host cell genome induced by Burkholderia pseudomallei reveals pathogen-specific and pathogen-generic signatures of infection. 2016, 6:30861 Sci Repen
dc.identifier.issn2045-2322-
dc.identifier.pmid27484700-
dc.identifier.doi10.1038/srep30861-
dc.identifier.urihttp://hdl.handle.net/11287/620271-
dc.description.abstractThe potential for epigenetic changes in host cells following microbial infection has been widely suggested, but few examples have been reported. We assessed genome-wide patterns of DNA methylation in human macrophage-like U937 cells following infection with Burkholderia pseudomallei, an intracellular bacterial pathogen and the causative agent of human melioidosis. Our analyses revealed significant changes in host cell DNA methylation, at multiple CpG sites in the host cell genome, following infection. Infection induced differentially methylated probes (iDMPs) showing the greatest changes in DNA methylation were found to be in the vicinity of genes involved in inflammatory responses, intracellular signalling, apoptosis and pathogen-induced signalling. A comparison of our data with reported methylome changes in cells infected with M. tuberculosis revealed commonality of differentially methylated genes, including genes involved in T cell responses (BCL11B, FOXO1, KIF13B, PAWR, SOX4, SYK), actin cytoskeleton organisation (ACTR3, CDC42BPA, DTNBP1, FERMT2, PRKCZ, RAC1), and cytokine production (FOXP1, IRF8, MR1). Overall our findings show that pathogenic-specific and pathogen-common changes in the methylome occur following infection.en
dc.language.isoenen
dc.publisherNatureen
dc.relation.urlhttp://dx.doi.org/10.1038/srep30861en
dc.rightsArchived with thanks to Scientific reportsen
dc.subjectWessex Classification Subject Headings::Oncology. Pathology.::Geneticsen
dc.titleMapping epigenetic changes to the host cell genome induced by Burkholderia pseudomallei reveals pathogen-specific and pathogen-generic signatures of infection.en
dc.typeJournal Articleen
dc.identifier.journalScientific reportsen
dc.description.noteThis article is freely available. Click on the Additional Link above to access the full-text.en
dc.type.versionPublisheden

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