Variation in 5-hydroxymethylcytosine across human cortex and cerebellum.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620277
Title:
Variation in 5-hydroxymethylcytosine across human cortex and cerebellum.
Authors:
Lunnon, K.; Hannon, E.; Smith, R. G.; Dempster, E. L.; Wong, C. C. Y.; Burrage, J.; Troakes, C.; Al-Sarraj, S.; Kepa, A.; Schalkwyk, L.; Mill, Jonathan
Abstract:
The most widely utilized approaches for quantifying DNA methylation involve the treatment of genomic DNA with sodium bisulfite; however, this method cannot distinguish between 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). Previous studies have shown that 5hmC is enriched in the brain, although little is known about its genomic distribution and how it differs between anatomical regions and individuals. In this study, we combine oxidative bisulfite (oxBS) treatment with the Illumina Infinium 450K BeadArray to quantify genome-wide patterns of 5hmC in two distinct anatomical regions of the brain from multiple individuals.
Citation:
Variation in 5-hydroxymethylcytosine across human cortex and cerebellum. 2016, 17:27 Genome Biol.
Publisher:
BioMed Central
Journal:
Genome biology
Issue Date:
16-Feb-2016
URI:
http://hdl.handle.net/11287/620277
DOI:
10.1186/s13059-016-0871-x
PubMed ID:
26883014
Additional Links:
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-0871-x
Note:
This article is freely available via Open Access. Click on the Additional Link above to access the full-text.
Type:
Journal Article
Language:
en
ISSN:
1474-760X
Appears in Collections:
Honorary contracts publications; 2016 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorLunnon, K.en
dc.contributor.authorHannon, E.en
dc.contributor.authorSmith, R. G.en
dc.contributor.authorDempster, E. L.en
dc.contributor.authorWong, C. C. Y.en
dc.contributor.authorBurrage, J.en
dc.contributor.authorTroakes, C.en
dc.contributor.authorAl-Sarraj, S.en
dc.contributor.authorKepa, A.en
dc.contributor.authorSchalkwyk, L.en
dc.contributor.authorMill, Jonathanen
dc.date.accessioned2017-03-13T10:44:45Z-
dc.date.available2017-03-13T10:44:45Z-
dc.date.issued2016-02-16-
dc.identifier.citationVariation in 5-hydroxymethylcytosine across human cortex and cerebellum. 2016, 17:27 Genome Biol.en
dc.identifier.issn1474-760X-
dc.identifier.pmid26883014-
dc.identifier.doi10.1186/s13059-016-0871-x-
dc.identifier.urihttp://hdl.handle.net/11287/620277-
dc.description.abstractThe most widely utilized approaches for quantifying DNA methylation involve the treatment of genomic DNA with sodium bisulfite; however, this method cannot distinguish between 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). Previous studies have shown that 5hmC is enriched in the brain, although little is known about its genomic distribution and how it differs between anatomical regions and individuals. In this study, we combine oxidative bisulfite (oxBS) treatment with the Illumina Infinium 450K BeadArray to quantify genome-wide patterns of 5hmC in two distinct anatomical regions of the brain from multiple individuals.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttps://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-0871-xen
dc.rightsArchived with thanks to Genome biologyen
dc.subjectWessex Classification Subject Headings::Oncology. Pathology.::Geneticsen
dc.titleVariation in 5-hydroxymethylcytosine across human cortex and cerebellum.en
dc.typeJournal Articleen
dc.identifier.journalGenome biologyen
dc.description.noteThis article is freely available via Open Access. Click on the Additional Link above to access the full-text.en
dc.type.versionPublisheden

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