Exploring the effect of laser excitation wavelength on signal recovery with deep tissue transmission Raman spectroscopy.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620295
Title:
Exploring the effect of laser excitation wavelength on signal recovery with deep tissue transmission Raman spectroscopy.
Authors:
Ghita, A.; Matousek, P.; Stone, Nick
Abstract:
The aim of this research was to find the optimal Raman excitation wavelength to attain the largest possible sensitivity in deep Raman spectroscopy of breast tissue. This involved careful consideration of factors such as tissue absorption, scattering, fluorescence and instrument response function. The study examined the tissue absorption profile combined with Raman scattering and detection sensitivity at seven different, laser excitation wavelengths in the near infrared region of the spectrum. Several key scenarios in regards to the sample position within the tissue were examined. The highest Raman band visibility over the background ratio in respect to biological tissue provides the necessary information for determining the optimum laser excitation wavelength for deep tissue analysis using transmission Raman spectroscopy, including detection of breast calcifications. For thick tissues with a mix of protein and fat, such as breast tissue, 790-810 nm is concluded to be the optimum excitation wavelength for deep Raman measurements.
Citation:
Exploring the effect of laser excitation wavelength on signal recovery with deep tissue transmission Raman spectroscopy. 2016, 141 (20):5738-5746 Analyst
Publisher:
Royal Society of Chemistry
Journal:
The Analyst
Issue Date:
21-Oct-2016
URI:
http://hdl.handle.net/11287/620295
DOI:
10.1039/c6an00490c
PubMed ID:
27464358
Additional Links:
http://dx.doi.org/10.1039/c6an00490c
Type:
Journal Article
Language:
en
ISSN:
1364-5528
Appears in Collections:
Honorary contracts publications; 2016 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorGhita, A.en
dc.contributor.authorMatousek, P.en
dc.contributor.authorStone, Nicken
dc.date.accessioned2017-03-17T11:22:48Z-
dc.date.available2017-03-17T11:22:48Z-
dc.date.issued2016-10-21-
dc.identifier.citationExploring the effect of laser excitation wavelength on signal recovery with deep tissue transmission Raman spectroscopy. 2016, 141 (20):5738-5746 Analysten
dc.identifier.issn1364-5528-
dc.identifier.pmid27464358-
dc.identifier.doi10.1039/c6an00490c-
dc.identifier.urihttp://hdl.handle.net/11287/620295-
dc.description.abstractThe aim of this research was to find the optimal Raman excitation wavelength to attain the largest possible sensitivity in deep Raman spectroscopy of breast tissue. This involved careful consideration of factors such as tissue absorption, scattering, fluorescence and instrument response function. The study examined the tissue absorption profile combined with Raman scattering and detection sensitivity at seven different, laser excitation wavelengths in the near infrared region of the spectrum. Several key scenarios in regards to the sample position within the tissue were examined. The highest Raman band visibility over the background ratio in respect to biological tissue provides the necessary information for determining the optimum laser excitation wavelength for deep tissue analysis using transmission Raman spectroscopy, including detection of breast calcifications. For thick tissues with a mix of protein and fat, such as breast tissue, 790-810 nm is concluded to be the optimum excitation wavelength for deep Raman measurements.en
dc.language.isoenen
dc.publisherRoyal Society of Chemistryen
dc.relation.urlhttp://dx.doi.org/10.1039/c6an00490cen
dc.rightsArchived with thanks to The Analysten
dc.subjectWessex Classification Subject Headings::Clinical pathologyen
dc.titleExploring the effect of laser excitation wavelength on signal recovery with deep tissue transmission Raman spectroscopy.en
dc.typeJournal Articleen
dc.identifier.journalThe Analysten
dc.type.versionPublisheden
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