Using Raman spectroscopy to characterize biological materials.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620307
Title:
Using Raman spectroscopy to characterize biological materials.
Authors:
Butler, H. J.; Ashton, L.; Bird, B.; Cinque, G.; Curtis, K.; Dorney, J.; Esmonde-White, K.; Fullwood, N. J.; Gardner, B.; Martin-Hirsch, P. L.; Walsh, M. J.; McAinsh, M. R.; Stone, Nick; Martin, F. L.
Abstract:
Raman spectroscopy can be used to measure the chemical composition of a sample, which can in turn be used to extract biological information. Many materials have characteristic Raman spectra, which means that Raman spectroscopy has proven to be an effective analytical approach in geology, semiconductor, materials and polymer science fields. The application of Raman spectroscopy and microscopy within biology is rapidly increasing because it can provide chemical and compositional information, but it does not typically suffer from interference from water molecules. Analysis does not conventionally require extensive sample preparation; biochemical and structural information can usually be obtained without labeling. In this protocol, we aim to standardize and bring together multiple experimental approaches from key leaders in the field for obtaining Raman spectra using a microspectrometer. As examples of the range of biological samples that can be analyzed, we provide instructions for acquiring Raman spectra, maps and images for fresh plant tissue, formalin-fixed and fresh frozen mammalian tissue, fixed cells and biofluids. We explore a robust approach for sample preparation, instrumentation, acquisition parameters and data processing. By using this approach, we expect that a typical Raman experiment can be performed by a nonspecialist user to generate high-quality data for biological materials analysis.
Citation:
Using Raman spectroscopy to characterize biological materials. 2016, 11 (4):664-87 Nat Protoc
Publisher:
Nature
Journal:
Nature protocols
Issue Date:
Apr-2016
URI:
http://hdl.handle.net/11287/620307
DOI:
10.1038/nprot.2016.036
PubMed ID:
26963630
Additional Links:
http://dx.doi.org/10.1038/nprot.2016.036
Type:
Journal Article
Language:
en
ISSN:
1750-2799
Appears in Collections:
Honorary contracts publications; 2016 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorButler, H. J.en
dc.contributor.authorAshton, L.en
dc.contributor.authorBird, B.en
dc.contributor.authorCinque, G.en
dc.contributor.authorCurtis, K.en
dc.contributor.authorDorney, J.en
dc.contributor.authorEsmonde-White, K.en
dc.contributor.authorFullwood, N. J.en
dc.contributor.authorGardner, B.en
dc.contributor.authorMartin-Hirsch, P. L.en
dc.contributor.authorWalsh, M. J.en
dc.contributor.authorMcAinsh, M. R.en
dc.contributor.authorStone, Nicken
dc.contributor.authorMartin, F. L.en
dc.date.accessioned2017-03-17T12:20:24Z-
dc.date.available2017-03-17T12:20:24Z-
dc.date.issued2016-04-
dc.identifier.citationUsing Raman spectroscopy to characterize biological materials. 2016, 11 (4):664-87 Nat Protocen
dc.identifier.issn1750-2799-
dc.identifier.pmid26963630-
dc.identifier.doi10.1038/nprot.2016.036-
dc.identifier.urihttp://hdl.handle.net/11287/620307-
dc.description.abstractRaman spectroscopy can be used to measure the chemical composition of a sample, which can in turn be used to extract biological information. Many materials have characteristic Raman spectra, which means that Raman spectroscopy has proven to be an effective analytical approach in geology, semiconductor, materials and polymer science fields. The application of Raman spectroscopy and microscopy within biology is rapidly increasing because it can provide chemical and compositional information, but it does not typically suffer from interference from water molecules. Analysis does not conventionally require extensive sample preparation; biochemical and structural information can usually be obtained without labeling. In this protocol, we aim to standardize and bring together multiple experimental approaches from key leaders in the field for obtaining Raman spectra using a microspectrometer. As examples of the range of biological samples that can be analyzed, we provide instructions for acquiring Raman spectra, maps and images for fresh plant tissue, formalin-fixed and fresh frozen mammalian tissue, fixed cells and biofluids. We explore a robust approach for sample preparation, instrumentation, acquisition parameters and data processing. By using this approach, we expect that a typical Raman experiment can be performed by a nonspecialist user to generate high-quality data for biological materials analysis.en
dc.language.isoenen
dc.publisherNatureen
dc.relation.urlhttp://dx.doi.org/10.1038/nprot.2016.036en
dc.rightsArchived with thanks to Nature protocolsen
dc.subjectWessex Classification Subject Headings::Clinical pathologyen
dc.titleUsing Raman spectroscopy to characterize biological materials.en
dc.typeJournal Articleen
dc.identifier.journalNature protocolsen
dc.type.versionPublisheden

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