High-resolution FTIR imaging of colon tissues for elucidation of individual cellular and histopathological features.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620309
Title:
High-resolution FTIR imaging of colon tissues for elucidation of individual cellular and histopathological features.
Authors:
Nallala, J.; Lloyd, G. R.; Shepherd, N.A.; Stone, Nick
Abstract:
Novel technologies that could complement current histopathology based cancer diagnostic methods are under examination. In this endeavour mid-infrared spectroscopic imaging is a promising candidate that can provide valuable bio-molecular information from unstained cells and tissues in a rapid and a non-destructive manner. With this imaging technique, the biochemical information obtained from smaller areas of the tissues can be of clinical significance and hence the measured pixel size. Until recently it was difficult to obtain spectral data from pixels below around 5 microns square. High NA objectives have been utilised to reduce the ideal diffraction limit, enabling for the first time elucidation of subcellular features. In this context, the ability of high-resolution imaging, obtained using novel high-magnification optics retro-fitted onto a bench top FTIR imaging system, to characterise histopathological features in colonic tissues has been tested. Formalin fixed paraffin embedded colon tissues from three different pathologies were imaged directly using the conventional and the high-magnification imaging set-ups. To circumvent chemical de-paraffinization protocols, an extended multiplicative signal correction (EMSC) based electronic de-paraffinization was carried out on all the infrared images. Multivariate analysis of the high-magnification infrared imaging data showed a detailed information of the histological features of the colon tissue in comparison to conventional imaging. Furthermore, high-magnification imaging has enabled a label-free characterization of the mucin rich goblet cell features in an unprecedented manner. The current study demonstrates the applicability of high-magnification FTIR imaging to characterise complex tissues on a smaller scale that could be of clinical significance.
Citation:
High-resolution FTIR imaging of colon tissues for elucidation of individual cellular and histopathological features. 2016, 141 (2):630-9 Analyst
Publisher:
Royal Society of Chemistry
Journal:
The Analyst
Issue Date:
21-Jan-2016
URI:
http://hdl.handle.net/11287/620309
DOI:
10.1039/c5an01871d
PubMed ID:
26549223
Additional Links:
http://dx.doi.org/10.1039/c5an01871d
Type:
Journal Article
Language:
en
ISSN:
1364-5528
Appears in Collections:
Honorary contracts publications; 2016 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorNallala, J.en
dc.contributor.authorLloyd, G. R.en
dc.contributor.authorShepherd, N.A.en
dc.contributor.authorStone, Nicken
dc.date.accessioned2017-03-17T12:26:38Z-
dc.date.available2017-03-17T12:26:38Z-
dc.date.issued2016-01-21-
dc.identifier.citationHigh-resolution FTIR imaging of colon tissues for elucidation of individual cellular and histopathological features. 2016, 141 (2):630-9 Analysten
dc.identifier.issn1364-5528-
dc.identifier.pmid26549223-
dc.identifier.doi10.1039/c5an01871d-
dc.identifier.urihttp://hdl.handle.net/11287/620309-
dc.description.abstractNovel technologies that could complement current histopathology based cancer diagnostic methods are under examination. In this endeavour mid-infrared spectroscopic imaging is a promising candidate that can provide valuable bio-molecular information from unstained cells and tissues in a rapid and a non-destructive manner. With this imaging technique, the biochemical information obtained from smaller areas of the tissues can be of clinical significance and hence the measured pixel size. Until recently it was difficult to obtain spectral data from pixels below around 5 microns square. High NA objectives have been utilised to reduce the ideal diffraction limit, enabling for the first time elucidation of subcellular features. In this context, the ability of high-resolution imaging, obtained using novel high-magnification optics retro-fitted onto a bench top FTIR imaging system, to characterise histopathological features in colonic tissues has been tested. Formalin fixed paraffin embedded colon tissues from three different pathologies were imaged directly using the conventional and the high-magnification imaging set-ups. To circumvent chemical de-paraffinization protocols, an extended multiplicative signal correction (EMSC) based electronic de-paraffinization was carried out on all the infrared images. Multivariate analysis of the high-magnification infrared imaging data showed a detailed information of the histological features of the colon tissue in comparison to conventional imaging. Furthermore, high-magnification imaging has enabled a label-free characterization of the mucin rich goblet cell features in an unprecedented manner. The current study demonstrates the applicability of high-magnification FTIR imaging to characterise complex tissues on a smaller scale that could be of clinical significance.en
dc.language.isoenen
dc.publisherRoyal Society of Chemistryen
dc.relation.urlhttp://dx.doi.org/10.1039/c5an01871den
dc.rightsArchived with thanks to The Analysten
dc.subjectWessex Classification Subject Headings::Clinical pathologyen
dc.titleHigh-resolution FTIR imaging of colon tissues for elucidation of individual cellular and histopathological features.en
dc.typeJournal Articleen
dc.identifier.journalThe Analysten
dc.type.versionPublisheden

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