Bovine and degenerated human annulus fibrosus: a microstructural and micromechanical comparison.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620333
Title:
Bovine and degenerated human annulus fibrosus: a microstructural and micromechanical comparison.
Authors:
Vergari, C.; Chan, Daniel; Clarke, Andrew; Mansfield, J. C.; Meakin, J. R.; Winlove, P. C.
Abstract:
The complex structure of the annulus fibrosus is strongly related to its mechanical properties. Recent work showed that it is possible to observe the relative movement of fibre bundles in loaded cow tail annulus; the aim of this work was to describe and quantify annulus fibrosus micromechanics in degenerated human disc, and compare it with cow tail annulus, an animal model often used in the literature. Second harmonic generation was used to image the collagen matrix in twenty strips of annulus fibrosus harvested from intervertebral disc of seven patients undergoing surgery. Samples were loaded to 6% tensile strain in 1% steps. Elastic modulus was calculated from loading curves, and micromechanical strains were calculated from the images using custom software. The same protocol was applied to twenty strips of annulus harvested from cow tail discs. Significant morphological differences were found between human and cow tail samples, the most striking being the lack of collagen fibre crimp in the former. Fibres were also observed bending and running from one lamella to the other, forming a strong flexible interface. Interdigitation of fibre bundles was also present at this interface. Quantitative results show complex patterns of inter-bundle and inter-lamellar behaviour, with inter-bundle sliding being the main strain mechanism. Elastic modulus was similar between species, and it was not affected by the degree of degeneration. This work gives an insight into the complex structure and mechanical function of the annulus fibrosus, which should be accounted for in disc numerical modelling.
Citation:
Bovine and degenerated human annulus fibrosus: a microstructural and micromechanical comparison. 2017 Biomech Model Mechanobiol
Publisher:
Springer
Journal:
Biomechanics and modeling in mechanobiology
Issue Date:
4-Apr-2017
URI:
http://hdl.handle.net/11287/620333
DOI:
10.1007/s10237-017-0900-z
PubMed ID:
28378119
Additional Links:
https://dx.doi.org/10.1007/s10237-017-0900-z
Type:
Journal Article
Language:
en
ISSN:
1617-7940
Appears in Collections:
Exeter Spinal Surgery Unit; 2017 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorVergari, C.en
dc.contributor.authorChan, Danielen
dc.contributor.authorClarke, Andrewen
dc.contributor.authorMansfield, J. C.en
dc.contributor.authorMeakin, J. R.en
dc.contributor.authorWinlove, P. C.en
dc.date.accessioned2017-05-17T15:48:28Z-
dc.date.available2017-05-17T15:48:28Z-
dc.date.issued2017-04-04-
dc.identifier.citationBovine and degenerated human annulus fibrosus: a microstructural and micromechanical comparison. 2017 Biomech Model Mechanobiolen
dc.identifier.issn1617-7940-
dc.identifier.pmid28378119-
dc.identifier.doi10.1007/s10237-017-0900-z-
dc.identifier.urihttp://hdl.handle.net/11287/620333-
dc.description.abstractThe complex structure of the annulus fibrosus is strongly related to its mechanical properties. Recent work showed that it is possible to observe the relative movement of fibre bundles in loaded cow tail annulus; the aim of this work was to describe and quantify annulus fibrosus micromechanics in degenerated human disc, and compare it with cow tail annulus, an animal model often used in the literature. Second harmonic generation was used to image the collagen matrix in twenty strips of annulus fibrosus harvested from intervertebral disc of seven patients undergoing surgery. Samples were loaded to 6% tensile strain in 1% steps. Elastic modulus was calculated from loading curves, and micromechanical strains were calculated from the images using custom software. The same protocol was applied to twenty strips of annulus harvested from cow tail discs. Significant morphological differences were found between human and cow tail samples, the most striking being the lack of collagen fibre crimp in the former. Fibres were also observed bending and running from one lamella to the other, forming a strong flexible interface. Interdigitation of fibre bundles was also present at this interface. Quantitative results show complex patterns of inter-bundle and inter-lamellar behaviour, with inter-bundle sliding being the main strain mechanism. Elastic modulus was similar between species, and it was not affected by the degree of degeneration. This work gives an insight into the complex structure and mechanical function of the annulus fibrosus, which should be accounted for in disc numerical modelling.en
dc.language.isoenen
dc.publisherSpringeren
dc.relation.urlhttps://dx.doi.org/10.1007/s10237-017-0900-zen
dc.rightsArchived with thanks to Biomechanics and modeling in mechanobiologyen
dc.subjectWessex Classification Subject Headings::Orthopaedicsen
dc.titleBovine and degenerated human annulus fibrosus: a microstructural and micromechanical comparison.en
dc.typeJournal Articleen
dc.identifier.journalBiomechanics and modeling in mechanobiologyen
dc.type.versionIn press (epub ahead of print)en

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