Microstructure and mechanics of human resistance arteries.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620401
Title:
Microstructure and mechanics of human resistance arteries.
Authors:
Bell, J S; Adio, A O; Pitt, A; Hayman, L; Thorn, C E; Shore, Angela ( 0000-0003-3039-308x ) ; Whatmore, J L; Winlove, C P
Abstract:
Vascular diseases such as diabetes and hypertension cause changes to the vasculature that can lead to vessel stiffening and the loss of vasoactivity. The microstructural bases of these changes are not presently fully understood. We present a new methodology for stain-free visualization, at a microscopic scale, of the morphology of the main passive components of the walls of unfixed resistance arteries and their response to changes in transmural pressure. Human resistance arteries were dissected from subcutaneous fat biopsies, mounted on a perfusion myograph, and imaged at varying transmural pressures using a multimodal nonlinear microscope. High-resolution three-dimensional images of elastic fibers, collagen, and cell nuclei were constructed. The honeycomb structure of the elastic fibers comprising the internal elastic layer became visible at a transmural pressure of 30 mmHg. The adventitia, comprising wavy collagen fibers punctuated by straight elastic fibers, thinned under pressure as the collagen network straightened and pulled taut. Quantitative measurements of fiber orientation were made as a function of pressure. A multilayer analytical model was used to calculate the stiffness and stress in each layer. The adventitia was calculated to be up to 10 times as stiff as the media and experienced up to 8 times the stress, depending on lumen diameter. This work reveals that pressure-induced reorganization of fibrous proteins gives rise to very high local strain fields and highlights the unique mechanical roles of both fibrous networks. It thereby provides a basis for understanding the micromechanical significance of structural changes that occur with age and disease.
Citation:
Microstructure and mechanics of human resistance arteries. 2016, 311 (6):H1560-H1568 Am. J. Physiol. Heart Circ. Physiol.
Publisher:
American Physiological Society
Journal:
American journal of physiology. Heart and circulatory physiology
Issue Date:
1-Dec-2016
URI:
http://hdl.handle.net/11287/620401
DOI:
10.1152/ajpheart.00002.2016
PubMed ID:
27663767
Additional Links:
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/27663767/
Note:
This article is freely available via Open Access. Click on the Additional Link above to access the full-text via the publisher's site.
Type:
Journal Article
Language:
en
ISSN:
1522-1539
Appears in Collections:
Cardiology; 2016 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorBell, J Sen
dc.contributor.authorAdio, A Oen
dc.contributor.authorPitt, Aen
dc.contributor.authorHayman, Len
dc.contributor.authorThorn, C Een
dc.contributor.authorShore, Angelaen
dc.contributor.authorWhatmore, J Len
dc.contributor.authorWinlove, C Pen
dc.date.accessioned2017-09-18T12:45:01Z-
dc.date.available2017-09-18T12:45:01Z-
dc.date.issued2016-12-01-
dc.identifier.citationMicrostructure and mechanics of human resistance arteries. 2016, 311 (6):H1560-H1568 Am. J. Physiol. Heart Circ. Physiol.en
dc.identifier.issn1522-1539-
dc.identifier.pmid27663767-
dc.identifier.doi10.1152/ajpheart.00002.2016-
dc.identifier.urihttp://hdl.handle.net/11287/620401-
dc.description.abstractVascular diseases such as diabetes and hypertension cause changes to the vasculature that can lead to vessel stiffening and the loss of vasoactivity. The microstructural bases of these changes are not presently fully understood. We present a new methodology for stain-free visualization, at a microscopic scale, of the morphology of the main passive components of the walls of unfixed resistance arteries and their response to changes in transmural pressure. Human resistance arteries were dissected from subcutaneous fat biopsies, mounted on a perfusion myograph, and imaged at varying transmural pressures using a multimodal nonlinear microscope. High-resolution three-dimensional images of elastic fibers, collagen, and cell nuclei were constructed. The honeycomb structure of the elastic fibers comprising the internal elastic layer became visible at a transmural pressure of 30 mmHg. The adventitia, comprising wavy collagen fibers punctuated by straight elastic fibers, thinned under pressure as the collagen network straightened and pulled taut. Quantitative measurements of fiber orientation were made as a function of pressure. A multilayer analytical model was used to calculate the stiffness and stress in each layer. The adventitia was calculated to be up to 10 times as stiff as the media and experienced up to 8 times the stress, depending on lumen diameter. This work reveals that pressure-induced reorganization of fibrous proteins gives rise to very high local strain fields and highlights the unique mechanical roles of both fibrous networks. It thereby provides a basis for understanding the micromechanical significance of structural changes that occur with age and disease.en
dc.language.isoenen
dc.publisherAmerican Physiological Societyen
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/27663767/en
dc.rightsArchived with thanks to American journal of physiology. Heart and circulatory physiologyen
dc.subjectWessex Classification Subject Headings::Cardiologyen
dc.titleMicrostructure and mechanics of human resistance arteries.en
dc.typeJournal Articleen
dc.identifier.journalAmerican journal of physiology. Heart and circulatory physiologyen
dc.description.noteThis article is freely available via Open Access. Click on the Additional Link above to access the full-text via the publisher's site.en
dc.type.versionPublisheden

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