Detection and localization of viral infection in the pancreas of patients with type 1 diabetes using short fluorescently-labelled oligonucleotide probes.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620419
Title:
Detection and localization of viral infection in the pancreas of patients with type 1 diabetes using short fluorescently-labelled oligonucleotide probes.
Authors:
Busse, N.; Paroni, F.; Richardson, S. J.; Laiho, J. E.; Oikarinen, M.; Frisk, G.; Hyöty, H.; de Koning, E.; Morgan, Noel; Maedler, K.
Abstract:
Enteroviruses, specifically of the Coxsackie B virus family, have been implicated in triggering islet autoimmunity and type 1 diabetes, but their presence in pancreata of patients with diabetes has not been fully confirmed.To detect the presence of very low copies of the virus genome in tissue samples from T1D patients, we designed a panel of fluorescently labeled oligonucleotide probes, each of 17-22 nucleotides in length with a unique sequence to specifically bind to the enteroviral genome of the picornaviridae family.With these probes enteroviral RNA was detected with high sensitivity and specificity in infected cells and tissues, including in FFPE pancreas sections from patients with T1D. Detection was not impeded by variations in sample processing and storage thereby overcoming the potential limitations of fragmented RNA. Co-staining of small RNA probes in parallel with classical immunstaining enabled virus detection in a cell-specific manner and more sensitively than by viral protein.
Citation:
Detection and localization of viral infection in the pancreas of patients with type 1 diabetes using short fluorescently-labelled oligonucleotide probes. 2017, 8 (8):12620-12636 Oncotarget
Publisher:
Oncotarget
Journal:
Oncotarget
Issue Date:
21-Feb-2017
URI:
http://hdl.handle.net/11287/620419
DOI:
10.18632/oncotarget.14896
PubMed ID:
28147344
Additional Links:
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28147344/
Note:
This article is freely available via Open Access. Click on the Additional Link above to access the full-text via the publisher's site.
Type:
Journal Article
Language:
en
ISSN:
1949-2553
Appears in Collections:
Honorary contracts publications; 2017 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorBusse, N.en
dc.contributor.authorParoni, F.en
dc.contributor.authorRichardson, S. J.en
dc.contributor.authorLaiho, J. E.en
dc.contributor.authorOikarinen, M.en
dc.contributor.authorFrisk, G.en
dc.contributor.authorHyöty, H.en
dc.contributor.authorde Koning, E.en
dc.contributor.authorMorgan, Noelen
dc.contributor.authorMaedler, K.en
dc.date.accessioned2017-10-04T13:16:11Z-
dc.date.available2017-10-04T13:16:11Z-
dc.date.issued2017-02-21-
dc.identifier.citationDetection and localization of viral infection in the pancreas of patients with type 1 diabetes using short fluorescently-labelled oligonucleotide probes. 2017, 8 (8):12620-12636 Oncotargeten
dc.identifier.issn1949-2553-
dc.identifier.pmid28147344-
dc.identifier.doi10.18632/oncotarget.14896-
dc.identifier.urihttp://hdl.handle.net/11287/620419-
dc.description.abstractEnteroviruses, specifically of the Coxsackie B virus family, have been implicated in triggering islet autoimmunity and type 1 diabetes, but their presence in pancreata of patients with diabetes has not been fully confirmed.To detect the presence of very low copies of the virus genome in tissue samples from T1D patients, we designed a panel of fluorescently labeled oligonucleotide probes, each of 17-22 nucleotides in length with a unique sequence to specifically bind to the enteroviral genome of the picornaviridae family.With these probes enteroviral RNA was detected with high sensitivity and specificity in infected cells and tissues, including in FFPE pancreas sections from patients with T1D. Detection was not impeded by variations in sample processing and storage thereby overcoming the potential limitations of fragmented RNA. Co-staining of small RNA probes in parallel with classical immunstaining enabled virus detection in a cell-specific manner and more sensitively than by viral protein.en
dc.language.isoenen
dc.publisherOncotargeten
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28147344/en
dc.rightsArchived with thanks to Oncotargeten
dc.subjectWessex Classification Subject Headings::Oncology. Pathology.::Geneticsen
dc.titleDetection and localization of viral infection in the pancreas of patients with type 1 diabetes using short fluorescently-labelled oligonucleotide probes.en
dc.typeJournal Articleen
dc.identifier.journalOncotargeten
dc.description.noteThis article is freely available via Open Access. Click on the Additional Link above to access the full-text via the publisher's site.en
dc.type.versionPublisheden

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