Regional differences in mitochondrial DNA methylation in human post-mortem brain tissue.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620434
Title:
Regional differences in mitochondrial DNA methylation in human post-mortem brain tissue.
Authors:
Devall, M.; Smith, R. G.; Jeffries, A. R.; Hannon, E.; Davies, M. N.; Schalkwyk, L.; Mill, Jonathan; Weedon, M. N.; Lunnon, K.
Abstract:
DNA methylation is an important epigenetic mechanism involved in gene regulation, with alterations in DNA methylation in the nuclear genome being linked to numerous complex diseases. Mitochondrial DNA methylation is a phenomenon that is receiving ever-increasing interest, particularly in diseases characterized by mitochondrial dysfunction; however, most studies have been limited to the investigation of specific target regions. Analyses spanning the entire mitochondrial genome have been limited, potentially due to the amount of input DNA required. Further, mitochondrial genetic studies have been previously confounded by nuclear-mitochondrial pseudogenes. Methylated DNA Immunoprecipitation Sequencing is a technique widely used to profile DNA methylation across the nuclear genome; however, reads mapped to mitochondrial DNA are often discarded. Here, we have developed an approach to control for nuclear-mitochondrial pseudogenes within Methylated DNA Immunoprecipitation Sequencing data. We highlight the utility of this approach in identifying differences in mitochondrial DNA methylation across regions of the human brain and pre-mortem blood.
Citation:
Regional differences in mitochondrial DNA methylation in human post-mortem brain tissue. 2017, 9:47 Clin Epigenetics
Publisher:
BioMed Central
Journal:
Clinical epigenetics
Issue Date:
May-2017
URI:
http://hdl.handle.net/11287/620434
DOI:
10.1186/s13148-017-0337-3
PubMed ID:
28473874
Additional Links:
https://clinicalepigeneticsjournal.biomedcentral.com/articles/10.1186/s13148-017-0337-3
Note:
This article is freely available via Open Access. Click on the Additional Link above to access the full-text via the publisher's site.
Type:
Journal Article
Language:
en
ISSN:
1868-7083
Appears in Collections:
Honorary contracts publications; 2017 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorDevall, M.en
dc.contributor.authorSmith, R. G.en
dc.contributor.authorJeffries, A. R.en
dc.contributor.authorHannon, E.en
dc.contributor.authorDavies, M. N.en
dc.contributor.authorSchalkwyk, L.en
dc.contributor.authorMill, Jonathanen
dc.contributor.authorWeedon, M. N.en
dc.contributor.authorLunnon, K.en
dc.date.accessioned2017-10-11T14:57:17Z-
dc.date.available2017-10-11T14:57:17Z-
dc.date.issued2017-05-
dc.identifier.citationRegional differences in mitochondrial DNA methylation in human post-mortem brain tissue. 2017, 9:47 Clin Epigeneticsen
dc.identifier.issn1868-7083-
dc.identifier.pmid28473874-
dc.identifier.doi10.1186/s13148-017-0337-3-
dc.identifier.urihttp://hdl.handle.net/11287/620434-
dc.description.abstractDNA methylation is an important epigenetic mechanism involved in gene regulation, with alterations in DNA methylation in the nuclear genome being linked to numerous complex diseases. Mitochondrial DNA methylation is a phenomenon that is receiving ever-increasing interest, particularly in diseases characterized by mitochondrial dysfunction; however, most studies have been limited to the investigation of specific target regions. Analyses spanning the entire mitochondrial genome have been limited, potentially due to the amount of input DNA required. Further, mitochondrial genetic studies have been previously confounded by nuclear-mitochondrial pseudogenes. Methylated DNA Immunoprecipitation Sequencing is a technique widely used to profile DNA methylation across the nuclear genome; however, reads mapped to mitochondrial DNA are often discarded. Here, we have developed an approach to control for nuclear-mitochondrial pseudogenes within Methylated DNA Immunoprecipitation Sequencing data. We highlight the utility of this approach in identifying differences in mitochondrial DNA methylation across regions of the human brain and pre-mortem blood.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttps://clinicalepigeneticsjournal.biomedcentral.com/articles/10.1186/s13148-017-0337-3en
dc.rightsArchived with thanks to Clinical epigeneticsen
dc.subjectWessex Classification Subject Headings::Oncology. Pathology.::Geneticsen
dc.titleRegional differences in mitochondrial DNA methylation in human post-mortem brain tissue.en
dc.typeJournal Articleen
dc.identifier.journalClinical epigeneticsen
dc.description.noteThis article is freely available via Open Access. Click on the Additional Link above to access the full-text via the publisher's site.en
dc.type.versionPublisheden

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