Developing Raman spectroscopy as a diagnostic tool for label-free antigen detection.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620437
Title:
Developing Raman spectroscopy as a diagnostic tool for label-free antigen detection.
Authors:
Lewis, A. T.; Gaifulina, R.; Guppy, N. J.; Isabelle, M.; Dorney, J.; Lloyd, G. R.; Rodriguez-Justo, M.; Kendall, C.; Stone, Nick; Thomas, G. M.
Abstract:
For several decades, a multitude of studies have documented the ability of Raman spectroscopy (RS) to differentiate between tissue types and identify pathological changes to tissues in a range of diseases. Furthermore, spectroscopists have illustrated that the technique is capable of detecting disease-specific alterations to tissue before morphological changes become apparent to the pathologist. This study draws comparisons between the information that is obtainable using RS alongside immunohistochemistry (IHC), since histological examination is the current GOLD standard for diagnosing a wide range of diseases. Here, Raman spectral maps were generated using formalin-fixed, paraffin-embedded colonic tissue sections from healthy patients and spectral signatures from principal components analysis (PCA) were compared with several IHC markers to confirm the validity of their localizations. PCA loadings identified a number of signatures that could be assigned to muscle, DNA and mucin glycoproteins and their distributions were confirmed with antibodies raised against anti-Desmin, anti-Ki67 and anti-MUC2, respectively. The comparison confirms that there is excellent correlation between RS and the IHC markers used, demonstrating that the technique is capable of detecting compositional changes in tissue in a label-free manner, eliminating the need for antibodies.
Citation:
Developing Raman spectroscopy as a diagnostic tool for label-free antigen detection. 2017 J Biophotonics
Publisher:
Wiley
Journal:
Journal of biophotonics
Issue Date:
12-Jul-2017
URI:
http://hdl.handle.net/11287/620437
DOI:
10.1002/jbio.201700028
PubMed ID:
28700142
Additional Links:
http://dx.doi.org/10.1002/jbio.201700028
Type:
Journal Article
Language:
en
ISSN:
1864-0648
Appears in Collections:
Honorary contracts publications; 2017 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorLewis, A. T.en
dc.contributor.authorGaifulina, R.en
dc.contributor.authorGuppy, N. J.en
dc.contributor.authorIsabelle, M.en
dc.contributor.authorDorney, J.en
dc.contributor.authorLloyd, G. R.en
dc.contributor.authorRodriguez-Justo, M.en
dc.contributor.authorKendall, C.en
dc.contributor.authorStone, Nicken
dc.contributor.authorThomas, G. M.en
dc.date.accessioned2017-10-11T15:08:39Z-
dc.date.available2017-10-11T15:08:39Z-
dc.date.issued2017-07-12-
dc.identifier.citationDeveloping Raman spectroscopy as a diagnostic tool for label-free antigen detection. 2017 J Biophotonicsen
dc.identifier.issn1864-0648-
dc.identifier.pmid28700142-
dc.identifier.doi10.1002/jbio.201700028-
dc.identifier.urihttp://hdl.handle.net/11287/620437-
dc.description.abstractFor several decades, a multitude of studies have documented the ability of Raman spectroscopy (RS) to differentiate between tissue types and identify pathological changes to tissues in a range of diseases. Furthermore, spectroscopists have illustrated that the technique is capable of detecting disease-specific alterations to tissue before morphological changes become apparent to the pathologist. This study draws comparisons between the information that is obtainable using RS alongside immunohistochemistry (IHC), since histological examination is the current GOLD standard for diagnosing a wide range of diseases. Here, Raman spectral maps were generated using formalin-fixed, paraffin-embedded colonic tissue sections from healthy patients and spectral signatures from principal components analysis (PCA) were compared with several IHC markers to confirm the validity of their localizations. PCA loadings identified a number of signatures that could be assigned to muscle, DNA and mucin glycoproteins and their distributions were confirmed with antibodies raised against anti-Desmin, anti-Ki67 and anti-MUC2, respectively. The comparison confirms that there is excellent correlation between RS and the IHC markers used, demonstrating that the technique is capable of detecting compositional changes in tissue in a label-free manner, eliminating the need for antibodies.en
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttp://dx.doi.org/10.1002/jbio.201700028en
dc.rightsArchived with thanks to Journal of biophotonicsen
dc.subjectWessex Classification Subject Headings::Biochemistryen
dc.titleDeveloping Raman spectroscopy as a diagnostic tool for label-free antigen detection.en
dc.typeJournal Articleen
dc.identifier.journalJournal of biophotonicsen
dc.type.versionPublisheden

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