Performance of mid infrared spectroscopy in skin cancer cell type identification

2.50
Hdl Handle:
http://hdl.handle.net/11287/620440
Title:
Performance of mid infrared spectroscopy in skin cancer cell type identification
Authors:
Kastl, L.; Kemper, B.; Lloyd, G. R.; Nallala, J.; Stone, Nick; Naranjo, V.; Penaranda, F.; Schnekenburger, J.
Abstract:
Marker free optical spectroscopy is a powerful tool for the rapid inspection of pathologically suspicious skin lesions and the non-invasive detection of early skin tumors. This goal can be reached by the combination of signal localization and the spectroscopical detection of chemical cell signatures. We here present the development and application of mid infrared spectroscopy (midIR) for the analysis of skin tumor cell types and three dimensional tissue phantoms towards the application of midIR spectroscopy for fast and reliable skin diagnostics. We developed standardized in vitro skin systems with increasing complexity, from single skin cell types as fibroblasts, keratinocytes and melanoma cells, to mixtures of these and finally three dimensional skin cancer phantoms. The cell systems were characterized with different systems in the midIR range up to 12 μm. The analysis of the spectra by novel data processing algorithms demonstrated the clear separation of all cell types, especially melanoma cells. Special attention and algorithm training was required for closely related mesenchymal cell types as dedifferentiated melanoma cells and fibroblasts. Proof of concept experiments with mixtures of in vivo fluorescence labelled skin cell types allowed the test of the new algorithms performance for the identification of specific cell types. The intense training of the software systems with various samples resulted in a increased sensitivity and specificity of the combined midIR and software system. These data highlight the potential of midIR spectroscopy as sensitive and specific future optical biopsy technology.
Citation:
Performance of mid infrared spectroscopy in skin cancer cell type identification. SPIE Proceedings Vol.10060, 2017.
Publisher:
SPIE
Journal:
SPIE Proceedings
Issue Date:
17-Feb-2017
URI:
http://hdl.handle.net/11287/620440
DOI:
10.1117/12.2253314
Additional Links:
http://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.2253314
Type:
Journal Article
Language:
en
Appears in Collections:
Honorary contracts publications; 2017 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorKastl, L.en
dc.contributor.authorKemper, B.en
dc.contributor.authorLloyd, G. R.en
dc.contributor.authorNallala, J.en
dc.contributor.authorStone, Nicken
dc.contributor.authorNaranjo, V.en
dc.contributor.authorPenaranda, F.en
dc.contributor.authorSchnekenburger, J.en
dc.date.accessioned2017-10-11T15:16:18Z-
dc.date.available2017-10-11T15:16:18Z-
dc.date.issued2017-02-17-
dc.identifier.citationPerformance of mid infrared spectroscopy in skin cancer cell type identification. SPIE Proceedings Vol.10060, 2017.en
dc.identifier.doi10.1117/12.2253314-
dc.identifier.urihttp://hdl.handle.net/11287/620440-
dc.description.abstractMarker free optical spectroscopy is a powerful tool for the rapid inspection of pathologically suspicious skin lesions and the non-invasive detection of early skin tumors. This goal can be reached by the combination of signal localization and the spectroscopical detection of chemical cell signatures. We here present the development and application of mid infrared spectroscopy (midIR) for the analysis of skin tumor cell types and three dimensional tissue phantoms towards the application of midIR spectroscopy for fast and reliable skin diagnostics. We developed standardized in vitro skin systems with increasing complexity, from single skin cell types as fibroblasts, keratinocytes and melanoma cells, to mixtures of these and finally three dimensional skin cancer phantoms. The cell systems were characterized with different systems in the midIR range up to 12 μm. The analysis of the spectra by novel data processing algorithms demonstrated the clear separation of all cell types, especially melanoma cells. Special attention and algorithm training was required for closely related mesenchymal cell types as dedifferentiated melanoma cells and fibroblasts. Proof of concept experiments with mixtures of in vivo fluorescence labelled skin cell types allowed the test of the new algorithms performance for the identification of specific cell types. The intense training of the software systems with various samples resulted in a increased sensitivity and specificity of the combined midIR and software system. These data highlight the potential of midIR spectroscopy as sensitive and specific future optical biopsy technology.en
dc.language.isoenen
dc.publisherSPIEen
dc.relation.urlhttp://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.2253314en
dc.subjectWessex Classification Subject Headings::Biochemistryen
dc.titlePerformance of mid infrared spectroscopy in skin cancer cell type identificationen
dc.typeJournal Articleen
dc.identifier.journalSPIE Proceedingsen
dc.type.versionPublisheden
dc.contributor.institutionWestfälische Wilhelms-Univ. Münster (Germany)-
dc.contributor.institutionWestfälische Wilhelms-Univ. Münster (Germany)-
dc.contributor.institutionGloucestershire Hospitals NHS Foundation Trust (United Kingdom)-
dc.contributor.institutionUniv. of Exeter (United Kingdom)-
dc.contributor.institutionUniv. of Exeter (United Kingdom)-
dc.contributor.institutionUniv. Politècnica de València (Spain)-
dc.contributor.institutionUniv. Politècnica de València (Spain)-
dc.contributor.institutionWestfälische Wilhelms-Univ. Münster (Germany)-
All Items in RD&E Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.