Detection of Aβ plaque-associated astrogliosis in Alzheimer's disease brain by spectroscopic imaging and immunohistochemistry.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620590
Title:
Detection of Aβ plaque-associated astrogliosis in Alzheimer's disease brain by spectroscopic imaging and immunohistochemistry.
Authors:
Palombo, F.; Tamagnini, F.; Jeynes, J. C.G.; Mattana, S.; Swift, I.; Nallala, J.; Hancock, J.; Brown, J. T.; Randall, A. D.; Stone, Nick
Abstract:
Recent work using micro-Fourier transform infrared (μFTIR) imaging has revealed that a lipid-rich layer surrounds many plaques in post-mortem Alzheimer's brain. However, the origin of this lipid layer is not known, nor is its role in the pathogenesis of Alzheimer's disease (AD). Here, we studied the biochemistry of plaques in situ using a model of AD. We combined FTIR, Raman and immunofluorescence images, showing that astrocyte processes co-localise with the lipid ring surrounding many plaques. We used μFTIR imaging to rapidly measure chemical signatures of plaques over large fields of view, and selected plaques for higher resolution analysis with Raman microscopy. Raman maps showed similar lipid rings and dense protein cores as in FTIR images, but also revealed cell bodies. We confirmed the presence of plaques using amylo-glo staining, and detected astrocytes using immunohistochemistry, revealing astrocyte co-localisation with lipid rings. This work is important because it correlates biochemical changes surrounding the plaque with the biological process of astrogliosis.
Citation:
Detection of Aβ plaque-associated astrogliosis in Alzheimer's disease brain by spectroscopic imaging and immunohistochemistry. 2017 Analyst
Publisher:
Royal Society of Chemistry
Journal:
The Analyst
Issue Date:
12-Dec-2017
URI:
http://hdl.handle.net/11287/620590
DOI:
10.1039/c7an01747b
PubMed ID:
29230441
Additional Links:
http://dx.doi.org/10.1039/c7an01747b
Type:
Journal Article
Language:
en
ISSN:
1364-5528
Appears in Collections:
Honorary contracts publications; 2017 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorPalombo, F.en
dc.contributor.authorTamagnini, F.en
dc.contributor.authorJeynes, J. C.G.en
dc.contributor.authorMattana, S.en
dc.contributor.authorSwift, I.en
dc.contributor.authorNallala, J.en
dc.contributor.authorHancock, J.en
dc.contributor.authorBrown, J. T.en
dc.contributor.authorRandall, A. D.en
dc.contributor.authorStone, Nicken
dc.date.accessioned2018-01-05T16:06:00Z-
dc.date.available2018-01-05T16:06:00Z-
dc.date.issued2017-12-12-
dc.identifier.citationDetection of Aβ plaque-associated astrogliosis in Alzheimer's disease brain by spectroscopic imaging and immunohistochemistry. 2017 Analysten
dc.identifier.issn1364-5528-
dc.identifier.pmid29230441-
dc.identifier.doi10.1039/c7an01747b-
dc.identifier.urihttp://hdl.handle.net/11287/620590-
dc.description.abstractRecent work using micro-Fourier transform infrared (μFTIR) imaging has revealed that a lipid-rich layer surrounds many plaques in post-mortem Alzheimer's brain. However, the origin of this lipid layer is not known, nor is its role in the pathogenesis of Alzheimer's disease (AD). Here, we studied the biochemistry of plaques in situ using a model of AD. We combined FTIR, Raman and immunofluorescence images, showing that astrocyte processes co-localise with the lipid ring surrounding many plaques. We used μFTIR imaging to rapidly measure chemical signatures of plaques over large fields of view, and selected plaques for higher resolution analysis with Raman microscopy. Raman maps showed similar lipid rings and dense protein cores as in FTIR images, but also revealed cell bodies. We confirmed the presence of plaques using amylo-glo staining, and detected astrocytes using immunohistochemistry, revealing astrocyte co-localisation with lipid rings. This work is important because it correlates biochemical changes surrounding the plaque with the biological process of astrogliosis.en
dc.language.isoenen
dc.publisherRoyal Society of Chemistryen
dc.relation.urlhttp://dx.doi.org/10.1039/c7an01747ben
dc.rightsArchived with thanks to The Analysten
dc.subjectWessex Classification Subject Headings::Neurology::Diseases & disorders of the nervous system (e.g. Parkinson's)en
dc.titleDetection of Aβ plaque-associated astrogliosis in Alzheimer's disease brain by spectroscopic imaging and immunohistochemistry.en
dc.typeJournal Articleen
dc.identifier.journalThe Analysten
dc.type.versionIn press (epub ahead of print)en

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