Sex-specific transcription and DNA methylation profiles of reproductive and epigenetic associated genes in the gonads and livers of breeding zebrafish.

2.50
Hdl Handle:
http://hdl.handle.net/11287/620848
Title:
Sex-specific transcription and DNA methylation profiles of reproductive and epigenetic associated genes in the gonads and livers of breeding zebrafish.
Authors:
Laing, L. V.; Viana, J.; Dempster, E. L.; Uren Webster, T. M.; van Aerle, R.; Mill, Jonathan; Santos, E M
Abstract:
Reproduction is an essential process for life and is regulated by complex hormone networks and environmental factors. To date, little is known about the contribution of epigenetic mechanisms to the regulation of reproduction, particularly in lower vertebrates. We used the zebrafish (Danio rerio) model to investigate the sex-specific transcription and DNA methylation profiles for genes involved in the regulation of reproduction and in epigenetic signalling in the livers and gonads. We found evidence for associations between DNA promotor methylation and transcription for esr1 (gonads and female livers), amh (gonads) and dnmt1 (livers). In the liver, esr1 was shown to be significantly over-expressed in females compared to males, and its promoter was significantly hypo-methylated in females compared to males. In the gonads, genes involved in epigenetic processes including dnmt1, dnmt3 and hdac1 were over-expressed in the ovary compared to the testis. In addition, dnmt1 and dnmt3 transcription in the testis was found to be strongly correlated with global DNA methylation. These data provide evidence of the sex-specific epigenetic regulation and transcription of genes involved in reproduction and epigenetic signalling in a commonly used vertebrate model.
Citation:
Sex-specific transcription and DNA methylation profiles of reproductive and epigenetic associated genes in the gonads and livers of breeding zebrafish. 2018, 222:16-25 Comp. Biochem. Physiol., Part A Mol. Integr. Physiol.
Publisher:
Elsevier
Journal:
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
Issue Date:
Aug-2018
URI:
http://hdl.handle.net/11287/620848
DOI:
10.1016/j.cbpa.2018.04.004
PubMed ID:
29655816
Additional Links:
https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(18)30053-9
Type:
Journal Article
Language:
en
ISSN:
1531-4332
Appears in Collections:
Honorary contracts publications; 2018 RD&E publications

Full metadata record

DC FieldValue Language
dc.contributor.authorLaing, L. V.en
dc.contributor.authorViana, J.en
dc.contributor.authorDempster, E. L.en
dc.contributor.authorUren Webster, T. M.en
dc.contributor.authorvan Aerle, R.en
dc.contributor.authorMill, Jonathanen
dc.contributor.authorSantos, E Men
dc.date.accessioned2018-10-04T09:50:08Z-
dc.date.available2018-10-04T09:50:08Z-
dc.date.issued2018-08-
dc.identifier.citationSex-specific transcription and DNA methylation profiles of reproductive and epigenetic associated genes in the gonads and livers of breeding zebrafish. 2018, 222:16-25 Comp. Biochem. Physiol., Part A Mol. Integr. Physiol.en
dc.identifier.issn1531-4332-
dc.identifier.pmid29655816-
dc.identifier.doi10.1016/j.cbpa.2018.04.004-
dc.identifier.urihttp://hdl.handle.net/11287/620848-
dc.description.abstractReproduction is an essential process for life and is regulated by complex hormone networks and environmental factors. To date, little is known about the contribution of epigenetic mechanisms to the regulation of reproduction, particularly in lower vertebrates. We used the zebrafish (Danio rerio) model to investigate the sex-specific transcription and DNA methylation profiles for genes involved in the regulation of reproduction and in epigenetic signalling in the livers and gonads. We found evidence for associations between DNA promotor methylation and transcription for esr1 (gonads and female livers), amh (gonads) and dnmt1 (livers). In the liver, esr1 was shown to be significantly over-expressed in females compared to males, and its promoter was significantly hypo-methylated in females compared to males. In the gonads, genes involved in epigenetic processes including dnmt1, dnmt3 and hdac1 were over-expressed in the ovary compared to the testis. In addition, dnmt1 and dnmt3 transcription in the testis was found to be strongly correlated with global DNA methylation. These data provide evidence of the sex-specific epigenetic regulation and transcription of genes involved in reproduction and epigenetic signalling in a commonly used vertebrate model.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S1095-6433(18)30053-9en
dc.rightsArchived with thanks to Comparative biochemistry and physiology. Part A, Molecular & integrative physiologyen
dc.subjectWessex Classification Subject Headings::Mental healthen
dc.titleSex-specific transcription and DNA methylation profiles of reproductive and epigenetic associated genes in the gonads and livers of breeding zebrafish.en
dc.typeJournal Articleen
dc.identifier.journalComparative biochemistry and physiology. Part A, Molecular & integrative physiologyen
dc.type.versionPublisheden

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